ArticleMicrobial cell factories2019
Development of a novel strategy for robust synthetic bacterial promoters based on a stepwise evolution targeting the spacer region of the core promoter in Bacillus subtilis.
Article in Microbial cell factories, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Engineering Bacillus subtilis for high-value bioproduction: recent advances and applications.Microbial cell factories · 2025Review
- Regulation of Riboflavin Biosynthesis in Microorganisms and Construction of the Advanced Overproducers of This Vitamin.International journal of molecular sciences · 2025Review
- Combining diffusion and transformer models for enhanced promoter synthesis and strength prediction in deep learning.mSystems · 2025Article
- Importance of the 5' untranslated region for recombinant enzyme production in isolated Bacillus subtilis 007.AMB Express · 2025Article
- Synthetic Homoserine Lactone Sensors for Gram-PositiveACS synthetic biology · 2024Article
- Exploring and Engineering Novel Strong Promoters for High-Level Protein Expression inMicroorganisms · 2023Article
- Prediction and validation of novel SigB regulon members in Bacillus subtilis and regulon structure comparison to Bacillales members.BMC microbiology · 2023Article
- Cis-Element Engineering Promotes the Expression ofInternational journal of molecular sciences · 2022Article
- Toward improved terpenoids biosynthesis: strategies to enhance the capabilities of cell factories.Bioresources and bioprocessing · 2022Review
- The multifunctionality of expression systems inExperimental biology and medicine (Maywood, N.J.) · 2021Review
- Importance of the 5' regulatory region to bacterial synthetic biology applications.Microbial biotechnology · 2021Review
- Intelligent host engineering for metabolic flux optimisation in biotechnology.The Biochemical journal · 2021Review
- An overview and future prospects of recombinant protein production in Bacillus subtilis.Applied microbiology and biotechnology · 2021Review
- Temperature effects on RNA polymerase initiation kinetics reveal which open complex initiates and that bubble collapse is stepwise.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Optimization of Cephalosporin C Acylase Expression in Escherichia coli by High-Throughput Screening a Constitutive Promoter Mutant library.Applied biochemistry and biotechnology · 2021Article
- Advances on systems metabolic engineering ofSynthetic and systems biotechnology · 2020Article
- Realization of Robust and Precise Regulation of Gene Expression by Multiple Sigma Recognizable Artificial Promoters.Frontiers in bioengineering and biotechnology · 2020Article
- Production of Vitamin B2 (Riboflavin) by Microorganisms: An Overview.Frontiers in bioengineering and biotechnology · 2020Review
- Efficient Overproduction of Active Nitrile Hydratase by Coupling Expression Induction and Enzyme MaturationFrontiers in bioengineering and biotechnology · 2020Article
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10 authors.
Funding
Abstract
backgroundPromoter evolution by synthetic promoter library (SPL) is a powerful approach to development of functional synthetic promoters to synthetic biology. However, it requires much tedious and time-consuming screenings because of the plethora of different variants in SPL. Actually, a large proportion of mutants in the SPL are significantly lower in strength, which contributes only to fabrication of a promoter library with a continuum of strength. Thus, to effectively obtain the evolved synthetic promoter exhibiting higher strength, it is essential to develop novel strategies to construct mutant library targeting the pivotal region rather than the arbitrary region of the template promoter. In this study, a strategy termed stepwise evolution targeting the spacer of core promoter (SETarSCoP) was established in Bacillus subtilis to effectively evolve the strength of bacterial promoter.
resultsThe native promoter, P
conclusionComparing with the commonly used SPL strategy, SETarSCoP facilitates the evolution process to obtain strength-evolved synthetic bacterial promoter through fabrication and screening of small-scale mutation libraries. This strategy will be a promising method to evolve diverse bacterial promoters to expand the toolbox for synthetic biology.
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