Evidence map›Paper›PMID 31142328›Full record

ArticleMalaria journal2019

Novel sporozoite-based ELISpot assay to assess frequency of parasite-specific B cells after vaccination with irradiated sporozoites.

Tanmaya Atre, Tanisha M Robinson, Tatyana Savransky, Sheetij Dutta, Judith E Epstein, Elke S Bergmann-Leitner

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Malaria journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.9field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Article
  3. ExploringFrontiers in immunology · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Tanmaya AtreMalaria Vaccine Branch, US Military Malaria Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Tanisha M RobinsonMalaria Vaccine Branch, US Military Malaria Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Tatyana SavranskyDivision of Entomology, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Sheetij DuttaMalaria Vaccine Branch, US Military Malaria Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Judith E EpsteinMalaria Department, Naval Medical Research Center, Silver Spring, MD, USA.
Elke S Bergmann-LeitnerMalaria Vaccine Branch, US Military Malaria Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA. elke.s.bergmann-leitner.civ@mail.mil.ORCID http://orcid.org/0000-0002-8571-8956
Walter Reed Army Institute of Research · USNaval Medical Research Command · US

Funding

Bill and Melinda Gates Foundation OPP1034596MIDRP Core Funding FCC
6 · The paper itself

Abstract

backgroundWhole parasite vaccination is an efficacious strategy to induce sterile immunity and to prevent malaria transmission. Understanding the mechanism and response of immune cells to vaccines plays a critical role in deciphering correlates of protection against infection and disease. Immunoassays, such as ELISpot, are commonly used to assess the immunogenicity of vaccines towards T cells and B cells. To date, these assays only analyse responses to specific antigens since they are based on recombinant parasite-derived proteins or peptides. There is the need for an agnostic approach that allows the evaluation of all sporozoite-associated antigens.

methodsELISpot plates coated with a defined amount of lysed Plasmodium falciparum sporozoites were used to assess the frequency of sporozoite-specific B cells in peripheral blood mononuclear cells from donors immunized with either a recombinant malaria vaccine or irradiated sporozoites.

resultsThis report describes the assay conditions for a specific and sensitive sporozoite-based B cell ELISpot assay. The assay development considers the quality of sporozoite preparation as well as the detection threshold of the frequency of antigen-specific B cells. The assay enables the detection of sporozoite-specific IgM and IgG-producing B cells. Moreover, the assay can detect sporozoite-reactive B cells from subjects that were either vaccinated with the radiation attenuated sporozoite vaccine or a recombinant pre-erythrocytic vaccine.

conclusionThe newly developed sporozoite-based B cell ELISpot enables the monitoring of changes in the frequency of sporozoite-specific B cells. Applying this assay to assess the potency of vaccination regimens or seasonal changes in B cell populations from subjects residing in malaria-endemic areas will provide an opportunity to gain insight into immune mechanisms involved in protection and/or disease.

Indexed as

Enzyme-Linked Immunospot AssayAntibodies, ProtozoanAntigens, ProtozoanB-LymphocytesClinical Trials as TopicHumansLeukocytes, MononuclearMalaria, FalciparumMalaria VaccinesPlasmodium falciparumProtozoan ProteinsSensitivity and SpecificitySporozoitesVaccines, SyntheticAntibodies, ProtozoanAntigens, ProtozoanMalaria VaccinesProtozoan ProteinsVaccines, SyntheticAntibodiesB cellsELISpotImmunityMalariaSporozoiteWhole parasite vaccine

Identifiers

PMID31142328
PMCPMC6540377
OpenAlexW2947868703

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.