ArticleMicrobial biotechnology2019
Segregostat: a novel concept to control phenotypic diversification dynamics on the example of Gram-negative bacteria.
Article in Microbial biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed.
- Overriding Bioprocess Perturbations With a Cell-Machine Interface for Reliable Microbial Stress-Response Control.Microbial biotechnology · 2026Article
- Dynamic Robust Control of Microbial Communities Using Cybergenetics.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Application of process analytical technology for real-time monitoring of synthetic co-culture bioprocesses.Analytical and bioanalytical chemistry · 2025Review
- Monitoring of the Single-Cell Behavior of anBiotech (Basel (Switzerland)) · 2025Article
- Release of extracellular DNA byiScience · 2025Article
- Fitness cost associated with cell phenotypic switching drives population diversification dynamics and controllability.Nature communications · 2023Article
- Controlling microbial co-culture based on substrate pulsing can lead to stability through differential fitness advantages.PLoS computational biology · 2022Article
- Xylanase production by Thermobacillus xylanilyticus is impaired by population diversification but can be mitigated based on the management of cheating behavior.Microbial cell factories · 2022Article
- Advances in automated real-time flow cytometry for monitoring of bioreactor processes.Engineering in life sciences · 2022Review
- Exploiting Information and Control Theory for Directing Gene Expression in Cell Populations.Frontiers in microbiology · 2022Review
- An automated DIY framework for experimental evolution of Pseudomonas putida.Microbial biotechnology · 2021Article
- Measurement Techniques to Resolve and Control Population Dynamics of Mixed-Culture Processes.Trends in biotechnology · 2021Review
- Single-Cell Technologies to Understand the Mechanisms of Cellular Adaptation in Chemostats.Frontiers in bioengineering and biotechnology · 2020Article
- Segregostat: a novel concept to control phenotypic diversification dynamics on the example of Gram-negative bacteria.Microbial biotechnology · 2019Article
- The Rocky Road From Fed-Batch to Continuous Processing WithFrontiers in bioengineering and biotechnology · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Controlling and managing the degree of phenotypic diversification of microbial populations is a challenging task. This task not only requires detailed knowledge regarding diversification mechanisms but also advanced technical set-ups for the real-time analyses and control of population behaviour on single-cell level. In this work, set-up, design and operation of the so called segregostat are described which, in contrast to a traditional chemostat, allows the control of phenotypic diversification of microbial populations over time. Two exemplary case studies will be discussed, i.e. phenotypic diversification dynamics of Eschericia coli and Pseudomonas putida based on outer membrane permeabilization, emphasizing the applicability and versatility of the proposed approach. Upon nutrient limitation, cell population tends to diversify into several subpopulations exhibiting distinct phenotypic features (non-permeabilized and permeabilized cells). Online analysis leads to the determination of the ratio between cells in these two states, which in turn triggers the addition of glucose pulses in order to maintain a predefined diversification ratio. These results prove that phenotypic diversification can be controlled by means of defined pulse-frequency modulation within continuously running bioreactor set-ups. This lays the foundation for systematic studies, not only of phenotypic diversification but also for all processes where dynamics single-cell approaches are required, such as synthetic co-culture processes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.