ArticleMolecular biology of the cell2019
Fine structure and dynamics of EB3 binding zones on microtubules in fibroblast cells.
Article in Molecular biology of the cell, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 21 citations in OpenAlex.
- Reconfiguration of the Microtubule Network and Dynamics in Hypoxic Cancer Cells.Chemical & biomedical imaging · 2026Article
- Hydrolysis-deficient mosaic microtubules as faithful mimics of the GTP cap.Nature communications · 2025Article
- Studying Microtubule Dynamics in Human Neurons: Two-Dimensional Microtubule Tracing and Kymographs in iPSC- and SH-SY5Y-Derived Neurons for Tau Research.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Morphological and cytoskeleton changes in cells after EMT.Scientific reports · 2023Article
- Fidgetin interacting with microtubule end binding protein EB3 affects axonal regrowth in spinal cord injury.Neural regeneration research · 2023Article
- Phase separation of +TIP networks regulates microtubule dynamics.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Beyond the GTP-cap: Elucidating the molecular mechanisms of microtubule catastrophe.BioEssays : news and reviews in molecular, cellular and developmental biology · 2023Article
- A kinesin-1 variant reveals motor-induced microtubule damage in cells.Current biology : CB · 2022Article
- Nucleus-cytoskeleton communication impacts on OCT4-chromatin interactions in embryonic stem cells.BMC biology · 2022Article
- On the Relationship Between EB-3 Profiles and Microtubules Growth in Cultured Cells.Frontiers in molecular biosciences · 2021Article
- Stress-induced phosphorylation of CLIP-170 by JNK promotes microtubule rescue.The Journal of cell biology · 2020Article
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Authors and funding
6 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
End-binding (EB) proteins associate with the growing tips of microtubules (MTs)and modulate their dynamics directly and indirectly, by recruiting essential factors to fine-tune MTs for their many essential roles in cells. Previously EB proteins have been shown to recognize a stabilizing GTP/GDP-Pi cap at the tip of growing MTs, but information about additional EB-binding zones on MTs has been limited. In this work, we studied fluorescence intensity profiles of one of the three mammalian EB-proteins, EB3, fused with red fluorescent protein (RFP). The distribution of EB3 on MTs in mouse fibroblasts frequently deviated from single exponential decay and exhibited secondary peaks. Those secondary peaks, which we refer to as EB3-islands, were detected on 56% comets of growing MTs and were encountered once per 44 s of EB3-RFP comet growth time with about 5 s half-lifetime. The majority of EB3-islands in the vicinity of MT tips was stationary and originated from EB3 comets moving with the growing MT tips. Computational modeling of the decoration of dynamic MT tips by EB3 suggested that the EB3-islands could not be explained simply by a stochastic first-order GTP hydrolysis/phosphate release. We speculate that additional protein factors contribute to EB3 residence time on MTs in cells, likely affecting MT dynamics.
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