ArticleAngewandte Chemie (International ed. in English)2019
A High-Throughput Mass Spectrometric Enzyme Activity Assay Enabling the Discovery of Cytochrome P450 Biocatalysts.
Article in Angewandte Chemie (International ed. in English), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- A novel P450 enzyme assay utilizing an NADPBiotechnology letters · 2025Article
- Choose Your Own Adventure: A Comprehensive Database of Reactions Catalyzed by Cytochrome P450 BM3 Variants.ACS catalysis · 2024Review
- Enabling technology and core theory of synthetic biology.Science China. Life sciences · 2023Review
- A phosphite-based screening platform for identification of enzymes favoring nonnatural cofactors.Scientific reports · 2022Article
- Directed evolution of a cyclodipeptide synthase with new activitiesChemical science · 2022Article
- Glycosyltransferases: Mining, engineering and applications in biosynthesis of glycosylated plant natural products.Synthetic and systems biotechnology · 2022Article
- A roadmap for metagenomic enzyme discovery.Natural product reports · 2021Review
- Faster, better, and cheaper: harnessing microfluidics and mass spectrometry for biotechnology.RSC chemical biology · 2021Review
- UPLC-MS/MS Method for Analysis of Endocannabinoid and Related Lipid Metabolism in Mouse Mucosal Tissue.Frontiers in physiology · 2021Article
- Perfluorocarbons in Chemical Biology.Chembiochem : a European journal of chemical biology · 2020Review
- Engineering natural and noncanonical nicotinamide cofactor-dependent enzymes: design principles and technology development.Current opinion in biotechnology · 2020Review
- Leveraging Oxidative Stress to Regulate Redox Balance-Based,ACS synthetic biology · 2020Article
- A Growth-Based, High-Throughput Selection Platform Enables Remodeling of 4-Hydroxybenzoate Hydroxylase Active Site.ACS catalysis · 2020Article
- An integrated screening system for the selection of exemplary substrates for natural and engineered cytochrome P450s.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Assaying for enzymatic activity is a persistent bottleneck in biocatalyst and drug development. Existing high-throughput assays for enzyme activity tend to be applicable only to a narrow range of biochemical transformations, whereas universal enzyme characterization methods usually require chromatography to determine substrate turnover, greatly diminishing throughput. We present an enzyme activity assay that allows the high-throughput mass-spectrometric detection of enzyme activity in complex matrices without the need for a chromatographic step. This technology, which we call probing enzymes with click-assisted NIMS (PECAN), can detect the activity of medically and biocatalytically significant cytochrome P450s in cell lysate, microsomes, and bacteria. Using this approach, a cytochrome P450
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Registered trials
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