Article3 Biotech2019
An overview of designing and selection of sgRNAs for precise genome editing by the CRISPR-Cas9 system in plants.
Article in 3 Biotech, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Validation of CRISPR construct activity and gene function in melon via a hairy root transformation system.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Article
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- Article
- Enhancing tiny millets through genome editing: current status and future prospects.Molecular genetics and genomics : MGG · 2025Review
- Recent advances in designing synthetic plant regulatory modules.Frontiers in plant science · 2025Review
- Article
- SiMul-db: a database of single and multi-target Cas9 guides for hazelnut editing.Frontiers in genetics · 2024Article
- Engineering CRISPR/Cas9 therapeutics for cancer precision medicine.Frontiers in genetics · 2024Review
- Iron oxide-coupled CRISPR-nCas9-based genome editing assessment in mucopolysaccharidosis IVA mice.Molecular therapy. Methods & clinical development · 2023Article
- Insights into Metabolic Engineering of Bioactive Molecules inCurrent genomics · 2023Review
- Guidelines for Performing CRISPR/Cas9 Genome Editing for Gene Validation and Trait Improvement in Crops.Plants (Basel, Switzerland) · 2023Review
- β-Catenin activity induces an RNA biosynthesis program promoting therapy resistance in T-cell acute lymphoblastic leukemia.EMBO molecular medicine · 2023Article
- Host-mediated gene engineering and microbiome-based technology optimization for sustainable agriculture and environment.Functional & integrative genomics · 2023Review
- Efficient CRISPR/Cas9 nickase-mediated genome editing in an in vitro model of mucopolysaccharidosis IVA.Gene therapy · 2023Article
- Multiplex CRISPR/Cas9 gene-editing platform in oil palm targeting mutations in EgFAD2 and EgPAT genes.Journal, genetic engineering & biotechnology · 2023Article
- CRISPR-mediated promoter editing of aFrontiers in genome editing · 2023Article
- The landscape of CRISPR/Cas9 for inborn errors of metabolism.Molecular genetics and metabolism · 2023Review
- CRISPR/Cas-based tools for the targeted control of plant viruses.Molecular plant pathology · 2022Review
- A sequential transformation method for validating soybean genome editing by CRISPR/Cas9 system.Saudi journal of biological sciences · 2022Article
- Strategies to overcome the main challenges of the use of CRISPR/Cas9 as a replacement for cancer therapy.Molecular cancer · 2022Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A large number of computational tools have been documented in recent years for identification of target-specific valid single-guide (sg) RNAs (18-20 nucleotide long sequence) that is an important component for the efficient utilization of the CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats-CRISPR-associated Protein) system. Despite optimization of Cas9, other major concerns are on-target efficiency and off-target activity that depend upon the sequence(s) of target-specific sgRNA(s). However, a very little attention has been paid for identification of the best-hit sgRNA for precise targeting as well as minimizing the off-target effects. The aim of this present work is to offer comparative insight into existing CRISPR software tools with their unique features (including targeted genome) and utilities. These available web tools were found to be designed based upon only a few limited mathematical models. Among all these available web tools, three (Benchling, Desktop and CRISPR-P) have been curated as exclusively available for plant genome-editing purpose. These three software tools have been comprehensively described and analyzed with single same target enquiry from two randomly selected genes (
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