Evidence map›Paper›PMID 31137900›Full record

ReviewInternational journal of molecular sciences2019

Selection of Biophysical Methods for Characterisation of Membrane Proteins.

Tristan O C Kwan, Rosana Reis, Giuliano Siligardi, Rohanah Hussain, Harish Cheruvara, Isabel Moraes

Abstract readReview
In one paragraph

Review in International journal of molecular sciences, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
  4. Review
  5. Structure and Function of Membrane Proteins.International journal of molecular sciences · 2023
    Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Review
  12. Review
  13. Review
  14. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Tristan O C KwanNational Physical Laboratory, Hampton Road, Teddington TW11 0LW, UK. tristan.kwan@npl.co.uk.ORCID 0000-0002-5524-6409
Rosana ReisNational Physical Laboratory, Hampton Road, Teddington TW11 0LW, UK. rosana.thevenot@npl.co.uk.
Giuliano SiligardiDiamond Light Source Ltd., Harwell Science and Innovation Campus, Didcot OX11 0DE, UK. giuliano.siligardi@diamond.ac.uk.ORCID 0000-0002-4667-6423
Rohanah HussainDiamond Light Source Ltd., Harwell Science and Innovation Campus, Didcot OX11 0DE, UK. rohanah.hussain@diamond.ac.uk.
Harish CheruvaraResearch Complex at Harwell, Rutherford Appleton Laboratory, Harwell Science and Innovation Campus, Didcot OX11 0FA, UK. harish.cheruvara@diamond.ac.uk.ORCID 0000-0001-7688-0401
Isabel MoraesNational Physical Laboratory, Hampton Road, Teddington TW11 0LW, UK. isabel.moraes@npl.co.uk.ORCID 0000-0002-7427-5467

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Over the years, there have been many developments and advances in the field of integral membrane protein research. As important pharmaceutical targets, it is paramount to understand the mechanisms of action that govern their structure-function relationships. However, the study of integral membrane proteins is still incredibly challenging, mostly due to their low expression and instability once extracted from the native biological membrane. Nevertheless, milligrams of pure, stable, and functional protein are always required for biochemical and structural studies. Many modern biophysical tools are available today that provide critical information regarding to the characterisation and behaviour of integral membrane proteins in solution. These biophysical approaches play an important role in both basic research and in early-stage drug discovery processes. In this review, it is not our objective to present a comprehensive list of all existing biophysical methods, but a selection of the most useful and easily applied to basic integral membrane protein research.

Indexed as

AnimalsCircular DichroismDynamic Light ScatteringFluorescence Recovery After PhotobleachingHumansMembrane ProteinsSpectrophotometry, InfraredMembrane Proteinsbiophysicscircular dichroismdynamic light scatteringLCP-FRAPmembrane proteinsMIRSEC-MALS

Identifiers

PMID31137900
PMCPMC6566885

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.