ReviewBioengineering (Basel, Switzerland)2019
Expansion Culture of Human Pluripotent Stem Cells and Production of Cardiomyocytes.
Review in Bioengineering (Basel, Switzerland), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed.
- Human cardiovascular organoids: Biomedical applications and ethical challenges.American heart journal plus : cardiology research and practice · 2026Review
- Cardiac-Derived ECM Microspheres for Enhanced hiPSC-CMs Maturation.Advanced functional materials · 2026Article
- Synthetic thermoresponsive scaffolds for the expansion and differentiation of human pluripotent stem cells into cardiomyocytes.RSC advances · 2025Article
- Cardiac organoids: a new tool for disease modeling and drug screening applications.Frontiers in cardiovascular medicine · 2025Review
- Reliability of high-quantity human brain organoids for modeling microcephaly, glioma invasion and drug screening.Nature communications · 2024Article
- Automated human induced pluripotent stem cell culture and sample preparation for 3D live-cell microscopy.Nature protocols · 2024Review
- Harnessing developmental cues for cardiomyocyte production.Development (Cambridge, England) · 2023Review
- Non-coding RNAs: key regulators of reprogramming, pluripotency, and cardiac cell specification with therapeutic perspective for heart regeneration.Cardiovascular research · 2022Review
- Rock inhibitor may compromise human induced pluripotent stem cells for cardiac differentiation in 3D.Bioactive materials · 2022Article
- Review
- Modeling Precision Cardio-Oncology: Using Human-Induced Pluripotent Stem Cells for Risk Stratification and Prevention.Current oncology reports · 2021Review
- Current Challenges and Solutions to Tissue Engineering of Large-scale Cardiac Constructs.Current cardiology reports · 2021Review
- Bioreactor Suspension Culture: Differentiation and Production of Cardiomyocyte Spheroids From Human Induced Pluripotent Stem Cells.Frontiers in bioengineering and biotechnology · 2021Article
- Progressive stretch enhances growth and maturation of 3D stem-cell-derived myocardium.Theranostics · 2021Article
- Selection of human induced pluripotent stem cells lines optimization of cardiomyocytes differentiation in an integrated suspension microcarrier bioreactor.Stem cell research & therapy · 2020Article
- Stem cell therapy of myocardial infarction: a promising opportunity in bioengineering.Advanced therapeutics · 2020Article
- Cell Therapy With Human ESC-Derived Cardiac Cells: Clinical Perspectives.Frontiers in bioengineering and biotechnology · 2020Review
- Human Brain Organoids to Decode Mechanisms of Microcephaly.Frontiers in cellular neuroscience · 2020Review
- Emerging Methods for Enhancing Pluripotent Stem Cell Expansion.Frontiers in cell and developmental biology · 2020Review
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transplantation of human pluripotent stem cell (hPSCs)-derived cardiomyocytes for the treatment of heart failure is a promising therapy. In order to implement this therapy requiring numerous cardiomyocytes, substantial production of hPSCs followed by cardiac differentiation seems practical. Conventional methods of culturing hPSCs involve using a 2D culture monolayer that hinders the expansion of hPSCs, thereby limiting their productivity. Advanced culture of hPSCs in 3D aggregates in the suspension overcomes the limitations of 2D culture and attracts immense attention. Although the hPSC production needs to be suitable for subsequent cardiac differentiation, many studies have independently focused on either expansion of hPSCs or cardiac differentiation protocols. In this review, we summarize the recent approaches to expand hPSCs in combination with cardiomyocyte differentiation. A comparison of various suspension culture methods and future prospects for dynamic culture of hPSCs are discussed in this study. Understanding hPSC characteristics in different models of dynamic culture helps to produce numerous cells that are useful for further clinical applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.