ArticlePloS one2019
Dietary multi-enzyme complex improves In Vitro nutrient digestibility and hind gut microbial fermentation of pigs.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
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Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it, 29 citations in OpenAlex.
- Meta-analysis identifies the effect of dietary multi-enzyme supplementation on gut health of pigs.Scientific reports · 2021Pooled it
- Liquid feeding of miscellaneous grain and meal diets with enzymes improved growth performance and intestinal health of growing pigs.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2026Article
- Managing Anti-Nutritional Factors in Plant-Based Feeds: Implications for Herbivore Nutrition and Production.Metabolites · 2026Review
- Effects of Matched Compound Enzyme on Nutrient Utilization and Physiological Responses in Growing Pigs Fed a Corn-Soybean Meal or Diversified Diet.Animals : an open access journal from MDPI · 2026Article
- Resource Utilization of Peanut Shells: Nutritional Characteristics, Regulation of Antinutritional Factors, and Application Potential in Livestock and Poultry Production.Food science & nutrition · 2025Review
- Anti-Nutritional Factors of Plant Protein Feeds for Ruminants and Methods for Their Elimination.Animals : an open access journal from MDPI · 2025Review
- Optimizing the zymogram of exogenous proteases in broiler diets by in vitro simulated gastrointestinal digestion and response surface methodology.Scientific reports · 2025Article
- Effects of Italian ryegrass with multi-enzymes supplementation on growth performance, gut microbial, and manure odor emission in finisher pig.Journal of animal science and technology · 2024Article
- Enzymatically hydrolyzed diet improves growth performance and intestinal microbiome in growing pigs.Frontiers in nutrition · 2024Article
- Xylanase improves the intestinal barrier function of Nile tilapia (Oreochromis niloticus) fed with soybean (Glycine max) meal.Journal of animal science and biotechnology · 2023Article
- Supplementation of a Multi-Carbohydrase and Phytase Complex in Diets Regardless of Nutritional Levels, Improved Nutrients Digestibility, Growth Performance, and Bone Mineralization of Growing-Finishing Pigs.Animals : an open access journal from MDPI · 2023Article
- A comparative study on in vitro and in vivo stomach-small intestinal and large intestinal digestion of plant protein meals in growing pigs.Journal of animal science · 2023Article
- Modulation of digestibility of canine food using enzyme supplement: anFrontiers in veterinary science · 2023Article
- Effects of protease enzyme supplementation in weanling pigs' diet with different crude protein levels on growth performance and nutrient digestibility.Journal of animal science and technology · 2022Article
- Residual Level, Histology, and Blood Biochemistry of Tebuconazole: A Repeated Dose 28-Day Oral Toxicity Study in Pigs.Food science of animal resources · 2022Article
- A systematic-review on the role of exogenous enzymes on the productive performance at weaning, growing and finishing in pigs.Veterinary and animal science · 2021Review
- Adaptation of Gut Microbiome to Transgenic Pigs Secreting β-Glucanase, Xylanase, and Phytase.Frontiers in genetics · 2021Article
- The transcriptome of the rumen ciliate Entodinium caudatum reveals some of its metabolic features.BMC genomics · 2019Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study was conducted in two stages to investigate the potential of multi-enzyme supplementation on the nutrient digestibility, growth performance, and gut microbial composition of pigs. In stage 1, effects of multi-enzyme complex (xylanase, α-amylase, β-glucanase, and protease) supplementation on the ileal and total tract dry matter (DM) digestibility of feed-stuffs were investigated with in vitro two-stage and three-stage enzyme incubation methods. A wide range of feed ingredients, namely, corn meal, wheat meal, soybean meal, fish meal, Oriental herbal extract, Italian rye-grass (IRG) and peanut hull were used as substrates. Supplementation of the multi-enzyme complex increased (P < 0.05) the digestibility of the Oriental herbal extract and corn meal. In stage 2, in vivo animal studies were performed to further investigate the effects of the dietary multi-enzyme complex on the nutrient utilization, growth performance, and fecal microbial composition of pigs. A total of 36 weaned pigs were fed corn- and soybean meal-based diets without (control) and with the multi-enzyme complex (treatment) for 6 weeks. Fecal samples were collected from 12 pigs to analyze the microbial communities by using DNA sequencing and bioinformatics tools. Multi-enzyme supplementation had no effect on apparent digestibility of nutrients and growth performance of pigs compared to control. Taxonomic analysis of the fecal samples indicated that the bacteria in both control and treatment samples predominantly belonged to Firmicutes and Bacteroidetes. In addition, the proportion of the phylum Firmicutes was slightly higher in the treatment group. At the genus level, the abundance of Treponema and Barnesiella increased in the treatment group; whereas the numbers ofthe genera including Prevotella, Butyricicoccus, Ruminococcus and Succinivibrio decreased in the treatment group. These results suggest that multi-enzyme supplementation with basal diets have the potential to improve nutrient digestibility and modify microbial communities in the hind-gut of pigs.
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