ArticleJournal of medical microbiology2019
Detection of respiratory pathogens in clinical samples using metagenomic shotgun sequencing.
Article in Journal of medical microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 22 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 51 citations in OpenAlex.
- Evaluating Seqstant LiveGene Analysis in real-time assessment of metagenomic next-generation sequencing (mNGS) data from respiratory samples.Infection · 2026Article
- Contrasting the gut microbiome in Colombian patients with diarrhea: a comparative metagenomic study in hospitalization and emergency room services.Gut pathogens · 2026Article
- Clinical pathogen profiles and lung microbiome features in lung infection patients and concurrent cancer: insights from metagenomics next-generation sequencing.Open life sciences · 2026Article
- Comparison of bronchoalveolar lavage fluid mNGS and sputum culture in the clinical application of severe pneumonia.Frontiers in cellular and infection microbiology · 2025Article
- Analysis of coinfections in patients with hematologic malignancies and COVID-19 by next-generation sequencing of bronchoalveolar lavage fluid.European journal of medical research · 2024Article
- Clinical and pathogen features of COVID-19-associated infections during an Omicron strain outbreak in Guangzhou, China.Microbiology spectrum · 2024Article
- Assessing the impact of triiodothyronine treatment on the lung microbiome of mice with pulmonary fibrosis.BMC pulmonary medicine · 2024Article
- Metabarcoding study of potential pathogens and zoonotic risks associated with dog feces in Seoul, South Korea.PLoS neglected tropical diseases · 2024Article
- PCR-Based Microarray Enhances Diagnosis of Culture-Negative Biopsied Tissue in Patients with Invasive Mold Infections: Real-World Experience in a Tertiary Medical Center.Journal of fungi (Basel, Switzerland) · 2024Article
- Clinical application of targeted next-generation sequencing in severe pneumonia: a retrospective review.Critical care (London, England) · 2024Article
- Association between Clinical Characteristics and Microbiota in Bronchiectasis Patients Based on Metagenomic Next-Generation Sequencing Technology.Polish journal of microbiology · 2024Article
- Review
- Pneumocystis jirovecii with high probability detected in bronchoalveolar lavage fluid of chemotherapy-related interstitial pneumonia in patients with lymphoma using metagenomic next-generation sequencing technology.Infectious agents and cancer · 2023Article
- Validation of a Metagenomic Next-Generation Sequencing Assay for Lower Respiratory Pathogen Detection.Microbiology spectrum · 2023Article
- Metagenomic next-generation sequencing of bronchoalveolar lavage fluid from children with severe pneumonia in pediatric intensive care unit.Frontiers in cellular and infection microbiology · 2023Article
- Functional lower airways genomic profiling of the microbiome to capture active microbial metabolism.The European respiratory journal · 2021Article
- Precision Pandemic Preparedness: Improving Diagnostics with Metagenomics.Journal of clinical microbiology · 2021Article
- Empirical Anti-MRSA vs Standard Antibiotic Therapy and Risk of 30-Day Mortality in Patients Hospitalized for Pneumonia.JAMA internal medicine · 2020Article
- Blood and Bronchoalveolar Lavage Fluid Metagenomic Next-Generation Sequencing in Pneumonia.The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2020Article
- Article
Corrections and comments
- Erratum issued
Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
purposeIn this pilot study, we used shotgun metagenome sequencing (SMS) strategy on bronchoalveolar lavage (BAL) samples from hospitalized patients with suspected ventilate-associated pneumonia (VAP) in order to explore its potential for improving detection of ventilator-associated-pneumonia (VAP) etiology. METHODOLOGY: In total, 67BAL samples from patients with VAP were tested with SMS strategy for detection of respiratory pathogens. Results of SMS and routine respiratory culture were compared.
resultsSMS detected all pathogens recovered by cultivation approaches. In addition, putative pathogens other than the organisms recovered by culture were detected by SMS in culture-positive samples. In 40 of 45 (89 %) culture-negative samples, a potential pathogen was detected by SMS.
conclusionThis proof-of-concept study demonstrates that SMS is able to detect bacterial, fungal and viral organisms in BAL, including culture-negative cases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.