ArticleRNA biology2019
CircNAPEPLD is expressed in human and murine spermatozoa and physically interacts with oocyte miRNAs.
Article in RNA biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.
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Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it, 40 citations in OpenAlex.
- Circular RNAs and Their Role in Male Infertility: A Systematic Review.Biomolecules · 2023Pooled it
- Methods for Investigating the Sperm Epigenome.Advances in experimental medicine and biology · 2026Review
- Epididymal-Born circRNA Cargo and Its Implications in Male Fertility.International journal of molecular sciences · 2025Review
- Sperm epigenetics and sperm RNAs as drivers of male infertility: truth or myth?Molecular and cellular biochemistry · 2025Review
- A comprehensive analysis of the inherited lncRNA and circRNA repertoire of zebrafish.Bioinformatics advances · 2025Article
- Circular RNAs: novel noncoding players in male infertility.Hereditas · 2024Review
- Variation of sperm quality and circular RNA content in men exposed to environmental contamination with heavy metals in 'Land of Fires', Italy.Human reproduction (Oxford, England) · 2024Article
- The Role of Circular RNAs in Male Infertility and Reproductive Cancers: A Narrative Review.Iranian journal of medical sciences · 2023Review
- Article
- Novel Insights into circRNA Saga Coming from Spermatozoa and Epididymis of HFD Mice.International journal of molecular sciences · 2023Article
- Antioxidants positively regulate obesity dependent circRNAs - sperm quality - functional axis.Frontiers in endocrinology · 2023Article
- Circular RNA CircSLC8A1 contributes to osteogenic differentiation in hBMSCs via CircSLC8A1/miR-144-3p/RUNX1 in periprosthetic osteolysis.Journal of cellular and molecular medicine · 2023Article
- circSMARCA5 Is an Upstream Regulator of the Expression of miR-126-3p, miR-515-5p, and Their mRNA Targets,International journal of molecular sciences · 2022Article
- The comprehensive detection of miRNA and circRNA in the regulation of intramuscular and subcutaneous adipose tissue of Laiwu pig.Scientific reports · 2022Article
- Article
- Competing endogenous RNA network mediated by circ_3205 in SARS-CoV-2 infected cells.Cellular and molecular life sciences : CMLS · 2022Article
- Actin remodeling driven by circLIMA1: sperm cell as an intriguing cellular model.International journal of biological sciences · 2022Article
- Identification of miRNAs Bound to an RNA of Interest by MicroRNA Capture Affinity Technology (miR-CATCH).Methods in molecular biology (Clifton, N.J.) · 2022Article
- FUS driven circCNOT6L biogenesis in mouse and human spermatozoa supports zygote development.Cellular and molecular life sciences : CMLS · 2021Article
- Article
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Authors and funding
16 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Circular RNAs (circRNAs) have a critical role in the control of gene expression. Their function in spermatozoa (SPZ) is unknown to date. Twenty-eight genes, involved in SPZ/testicular and epididymal physiology, were given in circBase database to find which of them may generate circular transcripts. We focused on circNAPEPLDiso1, one of the circular RNA isoforms of NAPEPLD transcript, because expressed in human and murine SPZ. In order to functionally characterize circNAPEPLDiso1 as potential microRNA (miRNA) sponge, we performed circNAPEPLDiso1-miR-CATCH and then profiled the expression of 754 miRNAs, by using TaqMan® Low Density Arrays. Among them, miRNAs 146a-5p, 203a-3p, 302c-3p, 766-3p and 1260a (some of them previously shown to be expressed in the oocyte), resulted enriched in circNAPEPLDiso1-miR-CATCHed cell lysate: the network of interactions generated from their validated targets was centred on a core of genes involved in the control of cell cycle. Moreover, computational analysis of circNAPEPLDiso1 sequence also showed its potential translation in a short form of NAPEPLD protein. Interestingly, the expression analysis in murine-unfertilized oocytes revealed low and high levels of circNAPEPLDiso1 and circNAPEPLDiso2, respectively. After fertilization, circNAPEPLDiso1 expression significantly increased, instead circNAPEPLDiso2 expression appeared constant. Based on these data, we suggest that SPZ-derived circNAPEPLDiso1 physically interacts with miRNAs primarily involved in the control of cell cycle; we hypothesize that it may represent a paternal cytoplasmic contribution to the zygote and function as a miRNA decoy inside the fertilized oocytes to regulate the first stages of embryo development. This role is proposed here for the first time.
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