Evidence map›Paper›PMID 31134591›Full record

ArticleGenes & genomics2019

Development and characterization of polymorphic microsatellite markers in northern fulmar, Fulmarus glacialis (Procellariiformes), and cross-species amplification in eight other seabirds.

Meg C Gravley, George K Sage, Andrew M Ramey, Scott A Hatch, Verena A Gill, Jolene R Rearick-Whitney, Aevar Petersen, Sandra L Talbot

Abstract read
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In one paragraph

Article in Genes & genomics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Meg C GravleyU. S. Geological Survey, Alaska Science Center, 4210 University Drive, Anchorage, AK, 99508, USA. mgravley@usgs.gov.ORCID 0000-0002-4947-0236
George K SageU. S. Geological Survey, Alaska Science Center, 4210 University Drive, Anchorage, AK, 99508, USA.ORCID 0000-0003-1431-2286
Andrew M RameyU. S. Geological Survey, Alaska Science Center, 4210 University Drive, Anchorage, AK, 99508, USA.ORCID 0000-0002-3601-8400
Scott A HatchU. S. Geological Survey, Alaska Science Center, 4210 University Drive, Anchorage, AK, 99508, USA.ORCID 0000-0002-0064-8187
Verena A GillNational Oceanic and Atmospheric Administration, National Marine Fisheries Service, 222 W. 7th Ave, Rm 552, Anchorage, AK, 99513, USA.ORCID 0000-0001-7289-1752
Jolene R Rearick-WhitneyU. S. Geological Survey, Alaska Science Center, 4210 University Drive, Anchorage, AK, 99508, USA.ORCID 0000-0003-0942-8268
Aevar Petersen, Brautarland 2, 108, Reykjavik, Iceland.ORCID 0000-0002-3977-1238
Sandra L TalbotU. S. Geological Survey, Alaska Science Center, 4210 University Drive, Anchorage, AK, 99508, USA.ORCID 0000-0002-3312-7214
United States Geological Survey · USNOAA National Marine Fisheries Service · USReykjavík Energy (Iceland) · IS

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundIn the North Pacific, northern fulmar (Fulmarus glacialis) forms extensive colonies in few locales, which may lead to limited gene flow and locale-specific population threats. In the Atlantic, there are thousands of colonies of varying sizes and in Europe the species is considered threatened. Prior screens and classical microsatellite development in fulmar failed to provide a suite of markers adequate for population genetics studies.

objectivesThe objective of this study was to isolate a suite of polymorphic microsatellite loci with sufficient variability to quantify levels of gene flow, population affinity, and identify familial relationships in fulmar. We also performed a cross-species screening of these markers in eight other species.

methodsWe used shotgun sequencing to isolate 26 novel microsatellite markers in fulmar to screen for variability using individuals from two distinct regions: the Pacific (Chagulak Island, Alaska) and the Atlantic (Hafnarey Island, Iceland).

resultsPolymorphism was present in 24 loci in Chagulak and 23 in Hafnarey, while one locus failed to amplify in either colony. Polymorphic loci exhibited moderate levels of genetic diversity and this suite of loci uncovered genetic structuring between the regions. Among the other species screened, polymorphism was present in one to seven loci.

conclusionThe loci yielded sufficient variability for use in population studies and estimation of familial relationships; as few as five loci provide resolution to determine individual identity. These markers will allow further insight into the global population dynamics and phylogeography of fulmars. We also demonstrated some markers are transferable to other species.

Indexed as

Microsatellite RepeatsPhylogenyPolymorphism, GeneticAnimalsBirdsEvolution, MolecularGene AmplificationGene FlowFulmarus glacialisMicrosatelliteNorthern fulmarShotgun sequencing

Identifiers

PMID31134591
OpenAlexW2947725189

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.