ArticleLife science alliance2019
Molecular characterization of CHAD domains as inorganic polyphosphate-binding modules.
Article in Life science alliance, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 42 citations in OpenAlex.
- Article
- Review
- Polyphosphate acts as an architectural regulator of carbon fixation and nucleoid structure in cyanobacteria.bioRxiv : the preprint server for biology · 2026Article
- Probing metazoan polyphosphate biology usingeLife · 2026Article
- Review
- Inorganic Polyphosphate and Human Diseases.Progress in molecular and subcellular biology · 2026Review
- Article
- An Update on Polyphosphate In Vivo Activities.Biomolecules · 2024Review
- Antipolyphosphate monoclonal antibodies derived from autoimmune mice.Research and practice in thrombosis and haemostasis · 2024Article
- The Protein Scaffolding Functions of Polyphosphate.Journal of molecular biology · 2024Review
- Possible Role of CHAD Proteins in Copper Resistance.Microorganisms · 2024Article
- Label-free biomolecular and cellular methods in small molecule epigallocatechin-gallate research.Heliyon · 2024Review
- Article
- Pull-Down Into Active Inclusion Bodies and Their Application in the Detection of (Poly)-Phosphates and Metal-Ions.Frontiers in bioengineering and biotechnology · 2022Article
- The Phosin PptA Plays a Negative Role in the Regulation of Antibiotic Production inAntibiotics (Basel, Switzerland) · 2021Article
- Grating-coupled interferometry reveals binding kinetics and affinities of Ni ions to genetically engineered protein layers.Scientific reports · 2020Article
- Acidocalcisomes and Polyphosphate Granules Are Different Subcellular Structures in Agrobacterium tumefaciens.Applied and environmental microbiology · 2020Article
- In Vitro Analytical Approaches to Study Plant Ligand-Receptor Interactions.Plant physiology · 2020Review
- Identity and functions of inorganic and inositol polyphosphates in plants.The New phytologist · 2020Review
- Inorganic Polyphosphates As Storage for and Generator of Metabolic Energy in the Extracellular Matrix.Chemical reviews · 2019Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Inorganic polyphosphates (polyPs) are linear polymers of orthophosphate units linked by phosphoanhydride bonds. Here, we report that bacterial, archaeal, and eukaryotic conserved histidine α-helical (CHAD) domains are specific polyP-binding modules. Crystal structures reveal that CHAD domains are formed by two four-helix bundles, giving rise to a central pore surrounded by conserved basic surface patches. Different CHAD domains bind polyPs with dissociation constants ranging from the nano- to mid-micromolar range, but not nucleic acids. A CHAD-polyP complex structure reveals the phosphate polymer binding across the central pore and along the two basic patches. Mutational analysis of CHAD-polyP interface residues validates the complex structure. The presence of a CHAD domain in the polyPase ygiF enhances its enzymatic activity. The only known CHAD protein from the plant
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