Evidence map›Paper›PMID 31132553›Full record

ArticleCarbohydrate research2019

Biochemical characterization of Helicobacter pylori α1-3-fucosyltransferase and its application in the synthesis of fucosylated human milk oligosaccharides.

Jing Bai, Zhigang Wu, Go Sugiarto, Madhusudhan Reddy Gadi, Hai Yu, Yanhong Li, Cong Xiao, Alice Ngo, Baohua Zhao, Xi Chen and 1 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Carbohydrate research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
2.4field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 30 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Review
  5. Article
  6. Article
  7. Review
  8. Enzymatic Synthesis of Glycans and Glycoconjugates.Advances in biochemical engineering/biotechnology · 2021
    Review
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 2 countries.

Jing BaiCollege of Life Science, Hebei Normal University, Shijiazhuang, Hebei, 050024, China.
Zhigang WuCollege of Bioscience and Bioengineering, Hebei University of Science and Technology, Shijiazhuang, Hebei, 050018, China.
Go SugiartoDepartment of Chemistry, University of California, One Shields Avenue, Davis, CA, 95616, USA.
Madhusudhan Reddy GadiDepartment of Chemistry, Center of Diagnostics & Therapeutics, Georgia State University, Atlanta, GA, 30303, USA.
Hai YuDepartment of Chemistry, University of California, One Shields Avenue, Davis, CA, 95616, USA.
Yanhong LiDepartment of Chemistry, University of California, One Shields Avenue, Davis, CA, 95616, USA.
Cong XiaoDepartment of Chemistry, Center of Diagnostics & Therapeutics, Georgia State University, Atlanta, GA, 30303, USA.
Alice NgoDepartment of Chemistry, University of California, One Shields Avenue, Davis, CA, 95616, USA.
Baohua ZhaoCollege of Life Science, Hebei Normal University, Shijiazhuang, Hebei, 050024, China. Electronic address: zhaobaohua@hebtu.edu.cn.
Xi ChenDepartment of Chemistry, University of California, One Shields Avenue, Davis, CA, 95616, USA. Electronic address: xiichen@ucdavis.edu.
Wanyi GuanCollege of Life Science, Hebei Normal University, Shijiazhuang, Hebei, 050024, China. Electronic address: guanwanyi@hebtu.edu.cn.
University of California, Davis · USHebei Normal University · CNGeorgia State University · USHebei University of Science and Technology · CN

Funding

Facile chemoenzymatic synthesis and purification of glycolipidsU01GM120419 · NIGMS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI CHEN, XI · 2016 to 2019
$2.8M
Oligosaccharides as antimicrobials and prebioticsR01HD065122 · NICHD · UNIVERSITY OF CALIFORNIA AT DAVIS · PI CHEN, XI · 2010 to 2013
$1.8M
NICHD NIH HHS R01 HD065122NIGMS NIH HHS U01 GM120419
6 · The paper itself

Abstract

Fucosylated human milk oligosaccharides (HMOs) have important biological functions. Enzymatic synthesis of such compounds requires robust fucosyltransferases. A C-terminal 66-amino acid truncated version of Helicobacter pylori α1-3-fucosyltransferase (Hp3FT) is a good candidate. Hp3FT was biochemically characterized to identify optimal conditions for enzymatic synthesis of fucosides. While N-acetyllactosamine (LacNAc) and lactose were both suitable acceptors, the former is preferred. At a low guanosine 5'-diphospho-β-L-fucose (GDP-Fuc) to acceptor ratio, Hp3FT selectively fucosylated LacNAc. Based on these enzymatic characteristics, diverse fucosylated HMOs, including 3-fucosyllactose (3-FL), lacto-N-fucopentaose (LNFP) III, lacto-N-neofucopentaose (LNnFP) V, lacto-N-neodifucohexaose (LNnDFH) II, difuco- and trifuco-para-lacto-N-neohexaose (DF-paraLNnH and TF-para-LNnH), were synthesized enzymatically by varying the ratio of the donor and acceptor as well as controlling the order of multiple glycosyltransferase-catalyzed reactions.

Indexed as

Chemistry Techniques, SyntheticFucoseFucosyltransferasesHelicobacter pyloriHumansHydrogen-Ion ConcentrationMetalsMilk, HumanOligosaccharidesFucoseFucosyltransferasesgalactoside 3-fucosyltransferaseMetalsOligosaccharidesEnzymatic synthesisFucosyltransferaseHuman milk oligosaccharideSubstrate specificity

Identifiers

PMID31132553
PMCPMC6613818
OpenAlexW2945880832

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.