ArticlePlant biotechnology journal2020
Pyrophosphate-fructose 6-phosphate 1-phosphotransferase (PFP1) regulates starch biosynthesis and seed development via heterotetramer formation in rice (Oryza sativa L.).
Article in Plant biotechnology journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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35 citing papers in PubMed, 63 citations in OpenAlex.
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- Identification of theFrontiers in genetics · 2026Article
- Comparative transcriptomics reveals differential carbohydrate and lipid metabolism in roots and nodules of Rhizobia-Inoculated alfalfa (Medicago sativa L.).BMC plant biology · 2025Article
- Extensin-like Protein OsPEX1 Modulates Grain Filling in Rice.Plants (Basel, Switzerland) · 2025Article
- Phenotypic characterization and genetic factor identification of starch content-related traits in foxtail millet (Setaria italica L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- The Endosperm-Specific GenePlants (Basel, Switzerland) · 2025Article
- Genome-wide identification of the phosphofructokinase gene family in maize (Zea mays L.) and their expression levels under abiotic stress and phytohormones.BMC plant biology · 2025Article
- A Dual-localized Fructose Bisphosphate Aldolase is Essential for Chloroplast Development and Carbon Metabolism in Rice.Rice (New York, N.Y.) · 2025Article
- Identification of a key locus, qRL8.1, associated with root length traits during seed germination under salt stress via a genome-wide association study in rice.BMC plant biology · 2025Article
- Allelic Expression Dynamics of Regulatory Factors During Embryogenic Callus Induction in ABB Banana (Plants (Basel, Switzerland) · 2025Article
- The LCG1-OsBP5/OsEBP89-Wx module regulates the grain chalkiness and taste quality in rice.Plant biotechnology journal · 2025Article
- Identification of theMolecular breeding : new strategies in plant improvement · 2025Article
- Comparative proteomic analysis of unfermented cocoa beans reveals key metabolic differences between fine-flavor and bulk genotypes.Frontiers in plant science · 2025Article
- RAL6 encodes a seed allergenic protein that positively regulates grain weight and seed germination.Journal of plant research · 2024Article
- Plant PR1 rescues condensation of the plastid iron-sulfur protein by a fungal effector.Nature plants · 2024Article
- Phenotypic Analysis and Gene Cloning of Rice Floury Endosperm MutantPlants (Basel, Switzerland) · 2024Article
- Source-sink synergy is the key unlocking sweet potato starch yield potential.Nature communications · 2024Article
- Genomic signatures of strawberry domestication and diversification.The Plant cell · 2024Article
- 'Autophagy · 2023Article
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Authors and funding
14 authors at 7 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pyrophosphate-fructose 6-phosphate 1-phosphotransferase (PFP1) reversibly converts fructose 6-phosphate and pyrophosphate to fructose 1, 6-bisphosphate and orthophosphate during glycolysis, and has diverse functions in plants. However, mechanisms underlying the regulation of starch metabolism by PFP1 remain elusive. This study addressed the function of PFP1 in rice floury endosperm and defective grain filling. Compared with the wild type, pfp1-3 exhibited remarkably low grain weight and starch content, significantly increased protein and lipid content, and altered starch physicochemical properties and changes in embryo development. Map-based cloning revealed that pfp1-3 is a novel allele and encodes the regulatory β-subunit of PFP1 (PFP1β). Measurement of nicotinamide adenine dinucleotide (NAD+) showed that mutation of PFP1β markedly decreased its enzyme activity. PFP1β and three of four putative catalytic α-subunits of PFP1, PFP1α1, PFP1α2, and PFP1α4, interacted with each other to form a heterotetramer. Additionally, PFP1β, PFP1α1 and PFP1α2 also formed homodimers. Furthermore, transcriptome analysis revealed that mutation of PFP1β significantly altered expression of many essential enzymes in starch biosynthesis pathways. Concentrations of multiple lipid and glycolytic intermediates and trehalose metabolites were elevated in pfp1-3 endosperm, indicating that PFP1 modulates endosperm metabolism, potentially through reversible adjustments to metabolic fluxes. Taken together, these findings provide new insights into seed endosperm development and starch biosynthesis and will help in the breeding of rice cultivars with higher grain yield and quality.
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