ArticleNucleic acids research2019
Maintenance of translational elongation rate underlies the survival of Escherichia coli during oxidative stress.
Article in Nucleic acids research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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40 citing papers in PubMed, 68 citations in OpenAlex.
- Bacterial stress responses lower mRNA-protein level correlations.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Regulating microbial cell factories to resist ROS stress in chemical production.Archives of microbiology · 2026Review
- Inhibition of ascorbic acid on Pseudomonas bubulae under super-chilled storage.World journal of microbiology & biotechnology · 2026Article
- Bacteriostatic Mechanism of the Ethyl Acetate Extract from the Root ofVeterinary sciences · 2026Article
- Resolving emergent transient oscillations in gene circuits with a growth-coupled model.Science advances · 2026Article
- Epitranscriptomic control of stress adaptations in Escherichia coli.Nucleic acids research · 2026Article
- Heterogeneity in responses to ribosome-targeting antibiotics mediated by bacterial RNA repair.Nature communications · 2025Article
- Investigation of the global translational response to oxidative stress in the model archaeonmSphere · 2025Article
- Photophysiological and transcriptomic response to the broad-spectrum herbicides atrazine and glyphosate in a photosynthetic picoeukaryote.Microbial genomics · 2025Article
- Proteomic Analysis ofFoods (Basel, Switzerland) · 2025Article
- Temporal changes in cold-inducible and uncharacterized Csps under heat and oxidative stress signify a role in bacterial stress response and adaptation.Archives of microbiology · 2025Article
- Study of the microenvironment of the lung flora in female lung adenocarcinoma patients: from benign lesions to invasive lung adenocarcinomas.BMC cancer · 2025Article
- PerR functions as a redox-sensing transcription factor regulating metal homeostasis in the thermoacidophilic archaeon Saccharolobus islandicus REY15A.Nucleic acids research · 2025Article
- Multi-kingdom microbial assemblage modulates its metabolism under contrasted cloud conditions.ISME communications · 2025Article
- The ability in managing reactive oxygen species affectsmSystems · 2024Article
- Selective 8-oxo-rG stalling occurs in the catalytic core of polynucleotide phosphorylase (PNPase) during degradation.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Article
- Unravelling the key steps impairing the metabolic state of Xanthomonas cells undergoing programmed cell death.International microbiology : the official journal of the Spanish Society for Microbiology · 2024Article
- Interplay between mistranslation and oxidative stress inArhiv za higijenu rada i toksikologiju · 2024Article
- Folding speeds of helical membrane proteins.Biochemical Society transactions · 2024Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To cope with harsh circumstances, bacterial cells must initiate cellular stress response programs, which demands the de novo synthesis of many stress defense proteins. Reactive oxygen species (ROS) is a universal environmental stressor for both prokaryotic cells and eukaryotic cells. However, the physiological burden that limits the survival of bacterial cells during oxidative stress remains elusive. Here we quantitatively characterize the cell growth and translational elongation rate of Escherichia coli cells treated with different doses of hydrogen peroxide. Cell growth is immediately arrested by low to moderate levels of hydrogen peroxide, but completely recovers after a certain lag time. The lag time depends positively on the dose of hydrogen peroxide. During the lag time, translational elongation rate drops by as much as ∼90% at initial stage and recovers to its normal state later, a phenomenon resulting from the dramatic alteration in cellular tRNA pools during oxidative stress. However, translational elongation is completely stalled at a certain threshold-level of hydrogen peroxide, at which cells ultimately fail to resume growth. Although the mRNA transcription of oxidative defense genes in oxyR regulon is dramatically induced upon hydrogen peroxide treatment, the extreme slow-down of translational elongation during high levels of hydrogen peroxide has severely compromised the timely synthesis of those oxidative defense proteins. Our study demonstrates that the tRNA-limited translational elongation is a key physiological bottleneck that the bacteria must overcome to counteract ROS, and the maintenance of translational elongation rate for timely synthesis of stress defense proteins is crucial for cells to smoothly get over the oxidative stress.
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