Evidence map›Paper›PMID 31131016›Full record

ArticlePlant methods2019

A high-throughput amplicon-based method for estimating outcrossing rates.

Friederike Jantzen, Natalia Wozniak, Christian Kappel, Adrien Sicard, Michael Lenhard

Abstract read
In one paragraph

Article in Plant methods, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Friederike Jantzen1Institute for Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Str. 24-25, House 26, 14476 Potsdam-Golm, Germany.
Natalia Wozniak1Institute for Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Str. 24-25, House 26, 14476 Potsdam-Golm, Germany.
Christian Kappel1Institute for Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Str. 24-25, House 26, 14476 Potsdam-Golm, Germany.
Adrien Sicard1Institute for Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Str. 24-25, House 26, 14476 Potsdam-Golm, Germany.
Michael Lenhard1Institute for Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Str. 24-25, House 26, 14476 Potsdam-Golm, Germany.ORCID 0000-0001-8661-6911

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe outcrossing rate is a key determinant of the population-genetic structure of species and their long-term evolutionary trajectories. However, determining the outcrossing rate using current methods based on PCR-genotyping individual offspring of focal plants for multiple polymorphic markers is laborious and time-consuming.

resultsWe have developed an amplicon-based, high-throughput enabled method for estimating the outcrossing rate and have applied this to an example of scented versus non-scented

conclusionsThis novel method for estimating outcrossing rates will allow determining this key population-genetic parameter with high-throughput across many genotypes in a population, enabling studies into the genetic determinants of successful pollinator attraction and outcrossing.

Indexed as

Amplicon sequencingCapsellaMixed matingOutcrossingOutcrossing rate

Identifiers

PMID31131016
PMCPMC6525360

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.