Evidence map›Paper›PMID 31130381›Full record

ArticleCell2019

A Cellular Taxonomy of the Bone Marrow Stroma in Homeostasis and Leukemia.

Ninib Baryawno, Dariusz Przybylski, Monika S Kowalczyk, Youmna Kfoury, Nicolas Severe, Karin Gustafsson, Konstantinos D Kokkaliaris, Francois Mercier, Marcin Tabaka, Matan Hofree and 9 more

Registry-linked trialOpen access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT04927611 (Single-cell Sequencing and Establishment of Cell Lines, Organoids, and Transplanted Tumor Models in Neuroendocrine Neoplasm), which is not on this map. Cited by 580 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
580citing papers in PubMed, 1 pooled it
126.0field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT04927611 unknown statusnot on this mapstarted 2021, after this paper: background citation

Single-cell Sequencing and Establishment of Cell Lines, Organoids, and Transplanted Tumor Models in Neuroendocrine Neoplasm

TypeobservationalSponsorFudan UniversityRan2021 to 2022Enrolled200ConditionsNeuroendocrine NeoplasmArmsBiopsy, open or laparoscopic surgery
3 · Its place in the literature

Who cites it

580 citing papers in PubMed, 1 synthesis or guideline pooled it, 892 citations in OpenAlex.

  1. Pooled it
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  15. A second wind for leukemic lungs.Nature immunology · 2026
    Article
  16. Article
  17. Targeting cellular senescence alleviates bone marrow aging.bioRxiv : the preprint server for biology · 2026
    Article
  18. Article
  19. Review
  20. Article

520 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

19 authors at 2 institutions in 3 countries.

Ninib BaryawnoCenter for Regenerative Medicine, Massachusetts General Hospital, Boston, MA 02114, USA; Harvard Stem Cell Institute, Cambridge, MA 02138, USA; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138, USA; Childhood Cancer Research Unit, Dep. of Children's and Women's Health, Karolinska Institutet, 171 77 Stockholm, Sweden.
Dariusz PrzybylskiKlarman Cell Observatory, Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA.
Monika S KowalczykKlarman Cell Observatory, Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA.
Youmna KfouryCenter for Regenerative Medicine, Massachusetts General Hospital, Boston, MA 02114, USA; Harvard Stem Cell Institute, Cambridge, MA 02138, USA; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138, USA.
Nicolas SevereCenter for Regenerative Medicine, Massachusetts General Hospital, Boston, MA 02114, USA; Harvard Stem Cell Institute, Cambridge, MA 02138, USA; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138, USA.
Karin GustafssonCenter for Regenerative Medicine, Massachusetts General Hospital, Boston, MA 02114, USA; Harvard Stem Cell Institute, Cambridge, MA 02138, USA; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138, USA.
Konstantinos D KokkaliarisCenter for Regenerative Medicine, Massachusetts General Hospital, Boston, MA 02114, USA; Harvard Stem Cell Institute, Cambridge, MA 02138, USA; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138, USA.
Francois MercierCenter for Regenerative Medicine, Massachusetts General Hospital, Boston, MA 02114, USA; Harvard Stem Cell Institute, Cambridge, MA 02138, USA; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138, USA.
Marcin TabakaKlarman Cell Observatory, Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA.
Matan HofreeKlarman Cell Observatory, Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA.
Danielle DionneKlarman Cell Observatory, Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA.
Ani PapazianCenter for Regenerative Medicine, Massachusetts General Hospital, Boston, MA 02114, USA; Harvard Stem Cell Institute, Cambridge, MA 02138, USA; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138, USA.
Dongjun LeeCenter for Regenerative Medicine, Massachusetts General Hospital, Boston, MA 02114, USA; Harvard Stem Cell Institute, Cambridge, MA 02138, USA; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138, USA; Department of Convergence Medical Science, Pusan National University School of Medicine, Yangsan 50612, Republic of Korea.
Orr AshenbergKlarman Cell Observatory, Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA.
Ayshwarya SubramanianKlarman Cell Observatory, Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA.
Eeshit Dhaval VaishnavKlarman Cell Observatory, Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA.
Orit Rozenblatt-RosenKlarman Cell Observatory, Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA.
Aviv RegevKlarman Cell Observatory, Broad Institute of Harvard and MIT, Cambridge, MA 02142, USA; Howard Hughes Medical Institute, Koch Institute of Integrative Cancer Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142, USA. Electronic address: aregev@broadinstitute.org.
David T ScaddenCenter for Regenerative Medicine, Massachusetts General Hospital, Boston, MA 02114, USA; Harvard Stem Cell Institute, Cambridge, MA 02138, USA; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138, USA. Electronic address: david_scadden@harvard.edu.
Broad Institute · USHarvard University · US

Funding

Center for Cell CircuitsRM1HG006193 · NHGRI · BROAD INSTITUTE, INC. · PI BLAINEY, PAUL CLARK, HACOHEN, NIR · 2016 to 2020
$14.9M
Deconvoluting the hematopoietic niche under stressR01DK107784 · NIDDK · MASSACHUSETTS GENERAL HOSPITAL · PI KHARCHENKO, PETER V, SCADDEN, DAVID T · 2016 to 2019
$2.1M
Howard Hughes Medical InstituteNHGRI NIH HHS RM1 HG006193NIDDK NIH HHS R01 DK107784
6 · The paper itself

Abstract

Stroma is a poorly defined non-parenchymal component of virtually every organ with key roles in organ development, homeostasis, and repair. Studies of the bone marrow stroma have defined individual populations in the stem cell niche regulating hematopoietic regeneration and capable of initiating leukemia. Here, we use single-cell RNA sequencing (scRNA-seq) to define a cellular taxonomy of the mouse bone marrow stroma and its perturbation by malignancy. We identified seventeen stromal subsets expressing distinct hematopoietic regulatory genes spanning new fibroblastic and osteoblastic subpopulations including distinct osteoblast differentiation trajectories. Emerging acute myeloid leukemia impaired mesenchymal osteogenic differentiation and reduced regulatory molecules necessary for normal hematopoiesis. These data suggest that tissue stroma responds to malignant cells by disadvantaging normal parenchymal cells. Our taxonomy of the stromal compartment provides a comprehensive bone marrow cell census and experimental support for cancer cell crosstalk with specific stromal elements to impair normal tissue function and thereby enable emergent cancer.

Indexed as

Cell DifferentiationHomeostasisOsteogenesisTumor MicroenvironmentAnimalsBone Marrow CellsHumansLeukemia, Myeloid, AcuteMiceOsteoblastsStromal Cellsbone marrow nichehematopoiesisleukemiasingle-cell RNA-sequencingstem cellstromatumor microenvironment

Identifiers

PMID31130381
PMCPMC6570562
OpenAlexW2946721812

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Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.