ArticleEndocrine2019
Mir-494 inhibits osteoblast differentiation by regulating BMP signaling in simulated microgravity.
Article in Endocrine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 38 citations in OpenAlex.
- TGF-β/BMP signaling in skeletal biology: molecular mechanisms, regulatory networks, and therapeutic implications in development, regeneration, and disease.Bone research · 2026Review
- Microrna Expression inMicroRNA (Shariqah, United Arab Emirates) · 2026Article
- The hierarchical stratum response system of organism to microgravity during spaceflight.Military Medical Research · 2026Review
- miR-144-3p targeting FLRT3 in osteogenic differentiation of mandibular bone marrow mesenchymal stem cells.Human genomics · 2025Article
- Overexpression of miR-671-3p alleviates postmenopausal osteoporosis by targeting GREM2 to activate BMP2/SMAD signaling pathway.Hereditas · 2025Article
- Non-coding RNAs mediate mechanical load-regulating bone metabolic homeostasis.Frontiers in cell and developmental biology · 2025Review
- Mechanical loading regulates osteogenic differentiation and bone formation by modulating non-coding RNAs.PeerJ · 2025Review
- [Research Progress in the Regulatory Role of circRNA-miRNA Network in Bone Remodeling].Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2024Review
- Article
- Post-Transcriptional Regulatory Crosstalk between MicroRNAs and Canonical TGF-β/BMP Signalling Cascades on Osteoblast Lineage: A Comprehensive Review.International journal of molecular sciences · 2023Review
- Protection of primary cilia is an effective countermeasure against the impairment of osteoblast function induced by simulated microgravity.Journal of cellular and molecular medicine · 2023Article
- Regulatory of miRNAs in tri-lineage differentiation of C3H10T1/2.Stem cell research & therapy · 2022Review
- The effects of microgravity on bone structure and function.NPJ microgravity · 2022Review
- Mouse Digit Tip Regeneration Is Mechanical Load Dependent.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2022Article
- A Quartet Network Analysis Identifying Mechanically Responsive Long Noncoding RNAs in Bone Remodeling.Frontiers in bioengineering and biotechnology · 2022Article
- Systemic effects of BMP2 treatment of fractures on non-injured skeletal sites during spaceflight.Frontiers in endocrinology · 2022Article
- The Inhibition of Osteoblast Viability by Monosodium Urate Crystal-Stimulated Neutrophil-Derived Exosomes.Frontiers in immunology · 2022Article
- Effects of Mechanical Stress Stimulation on Function and Expression Mechanism of Osteoblasts.Frontiers in bioengineering and biotechnology · 2022Review
- Long non-coding RNA MALAT1 enhances angiogenesis during bone regeneration by regulating the miR-494/SP1 axis.Laboratory investigation; a journal of technical methods and pathology · 2021Article
- Spaceflight Induced Disorders: Potential Nutritional Countermeasures.Frontiers in bioengineering and biotechnology · 2021Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Although the BMPR-SMAD-RUNX2 signaling pathway plays widely recognized roles in BMP-induced osteogenesis, factors regulating this pathway remain to be defined. In this study, we used simulated microgravity models, which represent mechanical unloading conditions, to detect miRNAs that function in osteoblast differentiation. We found that miR-494 was persistently increased in C2C12 cells subjected to clinorotation conditions and in osteoblasts isolated from tail-suspended rats. Experiments showed that the overexpression of miR-494 correlated with a marked reduction in osteoblast differentiation genes and a decrease in osteogenesis in BMP2-induced osteogenetic differentiation. In contrast, the inhibition of miR-494 promoted BMP2-induced osteogenesis and partially rescued osteoblast differentiation disorder under simulated microgravity conditions. Mechanism studies revealed that miR-494 directly targeted BMPR2 and RUNX2, both of which play vital roles in the BMPR-SMAD-RUNX2 signaling pathway. More importantly, we demonstrated a positive feedback loop between miR-494 and MYOD, a critical transcription factor for myogenesis, indicating that miR-494 may participate in deciding cell fate of the multipotent mesenchymal stem cells (MSCs). Collectively, our study reveals an important role for miR-494 in regulating osteogenesis, the identification of which not only clarifies a regulator of BMP2-induced osteoblast differentiation, but also offers a possible strategy for preventing bone loss under microgravity conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.