ArticleMolecular genetics and genomics : MGG2019
Efficient genome editing of Brassica campestris based on the CRISPR/Cas9 system.
Article in Molecular genetics and genomics : MGG, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Article
- Astaxanthin fromMarine drugs · 2025Review
- Tapping the microalgal potential: genetic precision and stress-induction for enhanced astaxanthin and biofuel production.Biotechnology for biofuels and bioproducts · 2025Review
- Gene editing of authenticHorticulture research · 2023Article
- Role ofHorticulture research · 2023Article
- CRISPR-mediated technology for seed oil improvement in rapeseed: Challenges and future perspectives.Frontiers in plant science · 2023Review
- Targeted genome editing in polyploids: lessons fromFrontiers in plant science · 2023Review
- The application of CRISPR/Cas technologies toaBIOTECH · 2022Review
- CRISPR/Cas9-mediated genome editing ofFrontiers in plant science · 2022Article
- CRISPR/Cas9 and Nanotechnology Pertinence in Agricultural Crop Refinement.Frontiers in plant science · 2022Review
- Improvement of glucosinolates by metabolic engineering inaBIOTECH · 2021Review
- Genome editing in fruit, ornamental, and industrial crops.Transgenic research · 2021Review
- Two Expansin Genes,Genes · 2021Article
- BcMF30a and BcMF30c, Two Novel Non-Tandem CCCH Zinc-Finger Proteins, Function in Pollen Development and Pollen Germination inInternational journal of molecular sciences · 2020Article
- Article
- Molecular characterization and expression analysis reveal the roles of CysPlant molecular biology · 2020Article
- CRISPR/Cas9 Genome Editing Technology: A Valuable Tool for Understanding Plant Cell Wall Biosynthesis and Function.Frontiers in plant science · 2020Review
- Genotype-Independent Transformation and Genome Editing ofFrontiers in plant science · 2020Article
- Development of Improved Fruit, Vegetable, and Ornamental Crops Using the CRISPR/Cas9 Genome Editing Technique.Plants (Basel, Switzerland) · 2019Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Conventional methods for gene function study in Brassica campestris have lots of drawbacks, which greatly hinder the identification of important genes' functions and molecular breeding. The clustered, regularly interspaced, short palindromic repeats (CRISPR) and CRISPR-associated protein 9 (CRISPR/Cas9) system is a versatile tool for genome editing that has been widely utilized in many plant species and has many advantages over conventional methods for gene function study. However, the application of CRISPR/Cas9 system in B. campestris remains unreported. The pectin-methylesterase genes Bra003491, Bra007665, and Bra014410 were selected as the targets of the CRISPR/Cas9 system. A single-targeting vector and a multitargeting vector were constructed. Different types of mutations were detected in T
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