ArticleAPMIS : acta pathologica, microbiologica, et immunologica Scandinavica2019
Obligate anaerobes are abundant in human necrotizing soft tissue infection samples - a metagenomics analysis.
Article in APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed, 26 citations in OpenAlex.
- Group B Streptococcus-Associated Necrotizing Soft Tissue Infection Following Minor Ankle Trauma: A Case Report.Cureus · 2026Article
- Article
- Consensus on the Diagnosis and Treatment of Adult Necrotizing Fasciitis (2025 Edition).Burns & trauma · 2025Article
- Is overweight a predictor for a more severe course of disease in cases of necrotizing fasciitis?European journal of trauma and emergency surgery : official publication of the European Trauma Society · 2024Article
- Development of a clinical metagenomics workflow for the diagnosis of wound infections.BMC medical genomics · 2024Article
- Rapid and precise identification of cervicothoracic necrotizing fasciitis caused byFrontiers in medicine · 2024Article
- The Skin and Gut Microbiome in Hidradenitis Suppurativa: Current Understanding and Future Considerations for Research and Treatment.American journal of clinical dermatology · 2022Review
- Isolation of obligate anaerobes from clinical samples received for routine bacterial culture and sensitivity: a cross sectional study.Iranian journal of microbiology · 2022Article
- Clinico-microbiological spectrum of anaerobic pyogenic infections in an Indian tertiary care teaching hospital: A two-year study.Journal of family medicine and primary care · 2021Article
- Clinical Implications of Polymicrobial Synergism Effects on Antimicrobial Susceptibility.Pathogens (Basel, Switzerland) · 2021Review
- Obligate anaerobes are abundant in human necrotizing soft tissue infection samples - a metagenomics analysis.APMIS : acta pathologica, microbiologica, et immunologica Scandinavica · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Necrotizing soft tissue infections (NSTIs) are associated with high morbidity and mortality and are increasing in incidence. Proper identification of the microbial causes of NSTIs is a crucial step in diagnosis and treatment, but the majority of data collected are culture based, which is biased against fastidious organisms, including obligate anaerobes. The goal of this study was to address this gap in knowledge by characterizing NSTI microbial communities through molecular diagnostics. We performed 16S rRNA sequencing on human NSTI samples and identified five genera most commonly found in NSTIs (Prevotella, Bacteroides, Peptoniphilus, Porphyromonas, and Enterococcus). We found that a >70% contribution of obligate anaerobes to the bacterial population distribution was associated with NSTI mortality, and that NSTI samples, from both survivors and non-survivors, had an increased relative abundance of gram negative bacteria compared to those of abscess patients. Based on our data, we conclude that obligate anaerobes are abundant in NSTIs and a high relative abundance of anaerobes is associated with a worse outcome. We recommend increasing anaerobe antibiotic coverage during the treatment of NSTIs even when anaerobes are not found by traditional clinical microbiology methods, and especially when there is a clinical suspicion for anaerobe involvement.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.