Evidence map›Paper›PMID 31127576›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2019

Chromatin Immunoprecipitation (ChIP) of Plasmid-Bound Proteins in Xenopus Egg Extracts.

Kelly B Wolfe, David T Long

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Kelly B WolfeDepartment of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC, USA.
David T LongDepartment of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC, USA. longdt@musc.edu.

Funding

Connecting BRCA1 functions with DNA crosslink sensitivityR35GM119512 · NIGMS · MEDICAL UNIVERSITY OF SOUTH CAROLINA · PI LONG, DAVID THOMAS · 2016 to 2025
$3.9M
Integrative Training in Oncogenic SignalingT32CA193201 · NCI · MEDICAL UNIVERSITY OF SOUTH CAROLINA · PI HOWE, PHILIP H, LONG, DAVID THOMAS · 2016 to 2025
$3.7M
NCI NIH HHS T32 CA193201NIGMS NIH HHS R35 GM119512
6 · The paper itself

Abstract

Xenopus egg extracts provide a cell-free system to analyze various aspects of chromatin biology. Here we describe a modified method of chromatin immunoprecipitation (ChIP) to detect the interaction of proteins with plasmid DNA incubated in extract. The combination of ChIP and Xenopus egg extracts provides a highly versatile and tractable approach to analyze dynamic protein-DNA interactions with great spatial and temporal detail.

Indexed as

AnimalsCell-Free SystemChromatinChromatin ImmunoprecipitationDNAOocytesPlasmidsProtein BindingSpatio-Temporal AnalysisXenopus laevisXenopus ProteinsChromatinDNAXenopus ProteinsCell-free systemChromatin immunoprecipitation (ChIP)DNA repairNucleoplasmic extractXenopus egg extract

Identifiers

PMID31127576
PMCPMC8091904

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.