Evidence map›Paper›PMID 31127558›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2019

Detection and Quantification of Lipid Droplets in Differentiated Human Podocytes.

Shamroop Kumar Mallela, Devang Maheshkumar Patel, Gloria Michelle Ducasa, Sandra Merscher, Alessia Fornoni, Hassan Al-Ali

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
6.0field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 16 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Shamroop Kumar MallelaKatz Family Division of Nephrology and Hypertension, Department of Medicine, University of Miami Miller School of Medicine, Miami, FL, USA.
Devang Maheshkumar PatelKatz Family Division of Nephrology and Hypertension, Department of Medicine, University of Miami Miller School of Medicine, Miami, FL, USA.
Gloria Michelle DucasaKatz Family Division of Nephrology and Hypertension, Department of Medicine, University of Miami Miller School of Medicine, Miami, FL, USA.
Sandra MerscherKatz Family Division of Nephrology and Hypertension, Department of Medicine, University of Miami Miller School of Medicine, Miami, FL, USA.
Alessia FornoniKatz Family Division of Nephrology and Hypertension, Department of Medicine, University of Miami Miller School of Medicine, Miami, FL, USA. afornoni@med.miami.edu.
Hassan Al-AliKatz Family Division of Nephrology and Hypertension, Department of Medicine, University of Miami Miller School of Medicine, Miami, FL, USA. halali@med.miami.edu.
University of Miami · US

Funding

Crosstalk Between Lipid Droplets and Other Organelles in PodocytesR01DK104753 · NIDDK · UNIVERSITY OF MIAMI SCHOOL OF MEDICINE · PI FORNONI, ALESSIA · 2015 to 2025
$5.9M
(PQ12) Targeting SMPDL3b to Prevent Radiation-Induced NephrotoxicityR01CA227493 · NCI · UNIVERSITY OF ROCHESTER · PI MARPLES, BRIAN · 2018 to 2022
$2.0M
Novel Targets of Rituximab in FSGSR01DK090316 · NIDDK · UNIVERSITY OF MIAMI SCHOOL OF MEDICINE · PI FORNONI, ALESSIA · 2011 to 2015
$1.7M
SMPDL3b and podocyte insulin signaling in DKDR01DK117599 · NIDDK · UNIVERSITY OF MIAMI SCHOOL OF MEDICINE · PI FORNONI, ALESSIA · 2018 to 2021
$1.4M
NCI NIH HHS R01 CA227493NIDDK NIH HHS R01 DK090316NIDDK NIH HHS R01 DK104753NIDDK NIH HHS R01 DK117599
6 · The paper itself

Abstract

Lipid droplets (LDs) are dynamic organelles that regulate the storage and homeostasis of intracellular triglycerides and other neutral lipids. Studies show that the number, morphology, and subcellular localization of LDs are altered in a growing number of diseases. As such, methodologies for imaging and quantifying LDs have become essential tools for detecting changes in cellular lipid metabolism, which could be an important indicator of disease onset or progression. We previously reported on the accumulation of LDs in podocytes of the kidney glomerulus in nephrological diseases of metabolic and non-metabolic origin. Here, we describe a high-content analysis (HCA) method for automated detection and quantification of LDs in differentiated human podocytes.

Indexed as

Cell Culture TechniquesCell DifferentiationCell LineFluorescent DyesHumansImage Processing, Computer-AssistedIntravital MicroscopyLipid DropletsLipid MetabolismLipidomicsMicroscopy, ConfocalPodocytesSoftwareFluorescent DyesCell-based assayHigh content screeningHuman podocytesLipid dropletsPhenotypic drug discovery

Identifiers

PMID31127558
PMCPMC7941729
OpenAlexW2946354535

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.