ArticleJournal of molecular neuroscience : MN2019
Potential Roles of miR-374a-5p in Mediating Neuroprotective Effects and Related Molecular Mechanism.
Article in Journal of molecular neuroscience : MN, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 11 citations in OpenAlex.
- Exploring extracellular vesicle surface markers and microRNA regulation following remote ischemic conditioning in patients with stroke; a randomized-controlled pilot study.BMC neuroscience · 2026Trial
- Circulating microRNA miR-425-5p Associated with Brain White Matter Lesions and Inflammatory Processes.International journal of molecular sciences · 2024Article
- Identification of EMP1 as a critical gene for cisplatin resistance in ovarian cancer by using integrated bioinformatics analysis.Cancer medicine · 2023Article
- MicroRNAs and Gene Regulatory Networks Related to Cleft Lip and Palate.International journal of molecular sciences · 2023Review
- miR-374a-5p alleviates sepsis-induced acute lung injury by targeting ZEB1 via the p38 MAPK pathway.Experimental and therapeutic medicine · 2022Article
- MicroRNA Expression in Pseudoexfoliation Syndrome with the Use of Next-Generation Sequencing.Genes · 2022Article
- Zinc Promotes Microglial Autophagy Through NLRP3 Inflammasome Inactivation via XIST/miR-374a-5p Axis in Spinal Cord Injury.Neurochemical research · 2022Article
- Umbilical cord blood troponin I, myoglobin and CK-MB in neonatal hypoxic ischemic encephalopathy and the clinical significance.Experimental and therapeutic medicine · 2020Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previous studies found that miR-374a-5p was decreased in infants suffering from the hypoxic-ischemic encephalopathy (HIE) compared with healthy control infants. However, the molecular mechanism of miR-374a-5p in the development of HIE remained unknown. This study is aimed to investigate the potential molecular pathway for shedding light on the treatment of HIE. An in vitro ischemia model in PC12 cells was established by oxygen/glucose deprivation (OGD). Reverse-transcription quantitative polymerase chain reaction and western blot were used to determine the levels of related genes or proteins in the OGD model or cells obtained from infants with HIE. Flow cytometry was conducted to quantify the apoptosis level of PC12 cells after OGD treatment. The TargetScan prediction algorithm was used to identify the potentially functional targets of miR-374a-5p. A dual-luciferase reporter assay was adopted to elucidate the sequences of miR-374a-5p binding to the 3'-UTR of potential target-PTEN. miR-374a-5p was downregulated in cells derived from human newborns with HIE, rat model with HIE, and PC12 cells after the OGD treatment. Inhibition of miR-374a-5p increased the expression of apoptotic markers and the apoptosis percentage of PC12 cells induced by OGD treatment while overexpression rescued the apoptosis. Meanwhile, PTEN expression was increased and suppressed after miR-374a-5p silence or overexpression, respectively. Upregulation of PTEN reversed the inhibitory effect of apoptotic markers elevation and PC12 cells apoptosis by the overexpression of miR-374a-5p after OGD treatment. PI3K pathway was required for the apoptosis effect caused by PTEN overexpression. We found that overexpression of miR-374a-5p reduced cell apoptosis through inhibiting PTEN/PI3K pathway in PC12 cells treated by OGD.
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