ArticleNucleic acids research2019
A group II intron-encoded protein interacts with the cellular replicative machinery through the β-sliding clamp.
Article in Nucleic acids research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 17 citations in OpenAlex.
- Structural Remodeling and Enzymatic Replacement Shape the Evolution of Organellar Group II Introns inInternational journal of molecular sciences · 2026Article
- Cysteine Mutagenesis of a Group II Intron-Encoded Protein Supports Splicing, Mobility, and Site-Specific Labeling.Biochemistry · 2025Article
- Article
- Presence of group II introns in phage genomes.Nucleic acids research · 2025Article
- Transposition with Tn3-family elements occurs through interaction with the host β-sliding clamp processivity factor.Nucleic acids research · 2024Article
- Identification of Group II Intron RmInt1 Binding Sites in a Bacterial Genome.Frontiers in molecular biosciences · 2022Article
- Distinct Expansion of Group II Introns During Evolution of Prokaryotes and Possible Factors Involved in Its Regulation.Frontiers in microbiology · 2022Article
- A new RNA-DNA interaction required for integration of group II intron retrotransposons into DNA targets.Nucleic acids research · 2021Article
- Prokaryotic reverse transcriptases: from retroelements to specialized defense systems.FEMS microbiology reviews · 2021Review
- Organellar Introns in Fungi, Algae, and Plants.Cells · 2021Review
- Methylation of rRNA as a host defense against rampant group II intron retrotransposition.Mobile DNA · 2021Article
- Bacterial Group II Intron Genomic Neighborhoods Reflect Survival Strategies: Hiding and Hijacking.Molecular biology and evolution · 2020Article
- Transitions between the steps of forward and reverse splicing of group IIC introns.RNA (New York, N.Y.) · 2020Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Group II introns are self-splicing mobile genetic retroelements. The spliced intron RNA and the intron-encoded protein (IEP) form ribonucleoprotein particles (RNPs) that recognize and invade specific DNA target sites. The IEP is a reverse transcriptase/maturase that may bear a C-terminal endonuclease domain enabling the RNP to cleave the target DNA strand to prime reverse transcription. However, some mobile introns, such as RmInt1, lack the En domain but nevertheless retrohome efficiently to transient single-stranded DNA target sites at a DNA replication fork. Their mobility is associated with host DNA replication, and they use the nascent lagging strand as a primer for reverse transcription. We searched for proteins that interact with RmInt1 RNPs and direct these RNPs to the DNA replication fork. Co-immunoprecipitation assays suggested that DnaN (the β-sliding clamp), a component of DNA polymerase III, interacts with the protein component of the RmInt1 RNP. Pulldown assays, far-western blots and biolayer interferometry supported this interaction. Peptide binding assays also identified a putative DnaN-interacting motif in the RmInt1 IEP structurally conserved in group II intron IEPs. Our results suggest that intron RNP interacts with the β-sliding clamp of the DNA replication machinery, favouring reverse splicing into the transient ssDNA at DNA replication forks.
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