ArticleThe Journal of antimicrobial chemotherapy2019
The mechanism of action of pepR, a viral-derived peptide, against Staphylococcus aureus biofilms.
Article in The Journal of antimicrobial chemotherapy, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 34 citations in OpenAlex.
- Article
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- Antimicrobial Peptides: Mechanisms, Applications, and Therapeutic Potential.Infection and drug resistance · 2025Review
- Expression and characterization of the new antimicrobial peptide AP138L-arg26 anti Staphylococcus aureus.Applied microbiology and biotechnology · 2024Article
- Liposomal Rifabutin-A Promising Antibiotic Repurposing Strategy against Methicillin-ResistantPharmaceuticals (Basel, Switzerland) · 2024Article
- In Silico and In vitro Evaluations of the Antibacterial Activities of HIV-1 Nef Peptides againstInternational journal of molecular and cellular medicine · 2024Article
- Quantitative Imaging of the Action of vCPP2319, an Antimicrobial Peptide from a Viral Scaffold, againstACS infectious diseases · 2023Article
- Linocin M18 protein from the insect pathogenic bacterium Brevibacillus laterosporus isolates.Applied microbiology and biotechnology · 2023Article
- The ribonuclease PNPase is a key regulator of biofilm formation in Listeria monocytogenes and affects invasion of host cells.NPJ biofilms and microbiomes · 2023Article
- The role of RNA regulators, quorum sensing and c-di-GMP in bacterial biofilm formation.FEBS open bio · 2023Review
- Acetate modulates the inhibitory effect ofMicrobial cell (Graz, Austria) · 2023Article
- A designed cyclic analogue of gomesin has potent activity against Staphylococcus aureus biofilms.The Journal of antimicrobial chemotherapy · 2022Article
- Assessment of New Strategies to Improve the Performance of Antimicrobial Peptides.Nanomaterials (Basel, Switzerland) · 2022Article
- Current treatments for biofilm-associated periprosthetic joint infection and new potential strategies.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2022Review
- Synergism between the Synthetic Antibacterial and Antibiofilm Peptide (SAAP)-148 and Halicin.Antibiotics (Basel, Switzerland) · 2022Article
- Four temporin-derived peptides exhibit antimicrobial and antibiofilm activities against methicillin-resistant.Acta biochimica et biophysica Sinica · 2022Article
- The Two Weapons against Bacterial Biofilms: Detection and Treatment.Antibiotics (Basel, Switzerland) · 2021Review
- PCL-1, a Trypsin-Resistant Peptide, Exerts Potent Activity Against Drug-Resistant Bacteria.Probiotics and antimicrobial proteins · 2021Article
- Liposomes as a Nanoplatform to Improve the Delivery of Antibiotics intoPharmaceutics · 2021Article
- Anti-Biofilm Effects of Synthetic Antimicrobial Peptides Against Drug-ResistantMolecules (Basel, Switzerland) · 2019Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesTo investigate the mechanism of action at the molecular level of pepR, a multifunctional peptide derived from the Dengue virus capsid protein, against Staphylococcus aureus biofilms.
methodsBiofilm mass, metabolic activity and viability were quantified using conventional microbiology techniques, while fluorescence imaging methods, including a real-time calcein release assay, were employed to investigate the kinetics of pepR activity at different biofilm depths.
resultsUsing flow cytometry-based assays, we showed that pepR is able to prevent staphylococcal biofilm formation due to a fast killing of planktonic bacteria, which in turn resulted from a peptide-induced increase in the permeability of the bacterial membranes. The activity of pepR against pre-formed biofilms was evaluated through the application of a quantitative live/dead confocal laser scanning microscopy (CLSM) assay. The results show that the bactericidal activity of pepR on pre-formed biofilms is dose and depth dependent. A CLSM-based assay of calcein release from biofilm-embedded bacteria was further developed to indirectly assess the diffusion and membrane permeabilization properties of pepR throughout the biofilm. A slower diffusion and delayed activity of the peptide at deeper layers of the biofilm were quantified.
conclusionsOverall, our results show that the activity of pepR on pre-formed biofilms is controlled by its diffusion along the biofilm layers, an effect that can be counteracted by an additional administration of peptide. Our study sheds new light on the antibiofilm mechanism of action of antimicrobial peptides, particularly the importance of their diffusion properties through the biofilm matrix on their activity.
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