ArticleScientific reports2019
Comparative analysis of silencing expression of myostatin (MSTN) and its two receptors (ACVR2A and ACVR2B) genes affecting growth traits in knock down chicken.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 44 citations in OpenAlex.
- Integrated Multi-Omic Analyses Reveal Gender-Specific Molecular Mechanisms Regulating Growth and Muscle Development in Zhongshan Partridge Ducks.Biomolecules · 2026Article
- Genome Editing in the Chicken: From PGC-Mediated Germline Transmission to Advanced Applications.International journal of molecular sciences · 2025Review
- Molecular mechanism of Activin receptor inhibition by DLK1.Nature communications · 2025Article
- Changes in Expression of Key Genes in Ceca of Chicken Broilers as Affected by Glyphosate, Antibiotics and a Coccidiostat.Animals : an open access journal from MDPI · 2024Article
- Myostatin gene role in regulating traits of poultry species for potential industrial applications.Journal of animal science and biotechnology · 2024Review
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- Use of AgomiR and AntagomiR technologies to alter satellite cell proliferationFrontiers in molecular biosciences · 2023Article
- Hyperpigmentation Inhibits Early Skeletal Muscle Development in Tengchong Snow Chicken Breed.Genes · 2022Article
- Silencing acetyl-CoA carboxylase A and sterol regulatory element-binding protein 1 genes through RNAi reduce serum and egg cholesterol in chicken.Scientific reports · 2022Article
- Embryonic transcriptome unravels mechanisms and pathways underlying embryonic development with respect to muscle growth, egg production, and plumage formation in native and broiler chickens.Frontiers in genetics · 2022Article
- Changes inAnimals : an open access journal from MDPI · 2021Article
- Article
- Transcriptome Analysis Reveals the Profile of Long Non-coding RNAs During Chicken Muscle Development.Frontiers in physiology · 2021Article
- Recent Advances in the Application of CRISPR/Cas9 Gene Editing System in Poultry Species.Frontiers in genetics · 2021Review
- Muscle differentiation induced by p53 signaling pathway-related genes in myostatin-knockout quail myoblasts.Molecular biology reports · 2020Article
- Similar sequences but dissimilar biological functions of GDF11 and myostatin.Experimental & molecular medicine · 2020Review
- Research Note: Association of temporal expression of myostatin with hypertrophic muscle growth in different Japanese quail lines.Poultry science · 2020Article
- Muscle Hyperplasia in Japanese Quail by Single Amino Acid Deletion in MSTN Propeptide.International journal of molecular sciences · 2020Article
- Post-transcriptional silencing of myostatin-1 in the spotted rose snapper (Lutjanus guttatus) promotes muscle hypertrophy.Molecular biology reports · 2020Article
- Exogenous Expression of an Alternative Splicing Variant of Myostatin Prompts Leg Muscle Fiber Hyperplasia in Japanese Quail.International journal of molecular sciences · 2019Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Myostatin (MSTN), a growth differentiation factor-8 regulates muscular development through its receptors, ACVR2A (Activin receptor type IIA) and ACVR2B (Activin receptor type IIB) by inhibiting cellular differentiation of developing somites during embryonic stage and diminishing myofibriller growth during post-embryonic period. The objective of this study was to compare the effect of knockdown of expression of myostatin, ACVR2A and ACVR2B genes on growth traits in chicken. The shRNAs for Myostatin, ACVR2A and ACVR2B genes were designed, synthesized and cloned in DEST vector. The recombinant molecules were transfected into the spermatozoa and transfected spermatozoa were inseminated artificially to the hens to obtain fertile eggs. The fertile eggs were collected, incubated in the incubator and hatched to chicks. Silencing of ACVR2B gene showed significantly higher body weight than other single, double and triple knock down of genes in transgenic birds. The carcass traits such as dressing%, leg muscle%, and breast muscle% were found with the highest magnitudes in birds with silencing of the ACVR2B gene as compared to the birds with that of other genes and control group. The lowest serum cholesterol and HDL content was found in ACVR2B silencing birds. The total RBC count was the highest in this group though the differential counts did not differ significantly among various silencing and control groups of birds. It is concluded that silencing of only one receptor of MSTN particularly, ACVR2B may augment the highest growth in chicken during juvenile stage. Our findings may be used as model for improving growth in other food animals and repairing muscular degenerative disorders in human and other animals.
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