ArticleScientific reports2019
A novel strategy to engineer pre-vascularized 3-dimensional skin substitutes to achieve efficient, functional engraftment.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 1 of them a synthesis that pooled it.
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The trial behind it
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Who cites it
33 citing papers in PubMed, 1 synthesis or guideline pooled it, 65 citations in OpenAlex.
- Impact of airborne particulate matter on skin: a systematic review from epidemiology to in vitro studies.Particle and fibre toxicology · 2020Pooled it
- A Roadmap to Perfused Skin: Defining the Next Generation of Research Questions in Cutaneous Tissue Engineering.International journal of molecular sciences · 2026Review
- Strategies and challenges in developing pre-vascularized three-dimensional skin substitutes using layer-by-layer cell coating technology.Regenerative therapy · 2026Article
- Human Subcutaneous Derived Stromal Vascular Fraction Endothelial Cells Display Venous and Arterial Markers in Culture and 3D Capillaries.Tissue engineering and regenerative medicine · 2026Article
- Pre-Vascularized 3-Dimensional Skin Substitutes Promote Angiogenesis and Tissue Repair in a Murine Model of Refractory Skin Ulcers.Journal of functional biomaterials · 2025Article
- Vascularized 3D Human Skin Models in the Forefront of Dermatological Research.Advanced healthcare materials · 2024Review
- Recent Developments in Layer-by-Layer Assembly for Drug Delivery and Tissue Engineering Applications.Advanced healthcare materials · 2024Review
- Advancements in bioengineered and autologous skin grafting techniques for skin reconstruction: a comprehensive review.Frontiers in bioengineering and biotechnology · 2024Article
- A review of the current state of natural biomaterials in wound healing applications.Frontiers in bioengineering and biotechnology · 2024Review
- FastSkinJournal of clinical medicine · 2023Article
- Combining Mesenchymal Stem Cells Derived from Wharton's Jelly and Amniotic Biomaterial Scaffolds for Cell Delivery.ACS omega · 2023Article
- Autologous Skin Grafts, versus Tissue-engineered Skin Constructs: A Systematic Review and Meta-analysis.Plastic and reconstructive surgery. Global open · 2023Article
- Engineered Vasculogenic Extracellular Vesicles Drive Nonviral Direct Conversions of Human Dermal Fibroblasts into Induced Endothelial Cells and Improve Wound Closure.Advanced therapeutics · 2023Article
- Engineering edgeless human skin with enhanced biomechanical properties.Science advances · 2023Article
- Leveraging Tissue Engineering for Skin Cancer Models.Advances in experimental medicine and biology · 2023Article
- Spongy-like hydrogels prevascularization with the adipose tissue vascular fraction delays cutaneous wound healing by sustaining inflammatory cell influx.Materials today. Bio · 2022Article
- Integrin-specific hydrogels for growth factor-free vasculogenesis.NPJ Regenerative medicine · 2022Article
- Proof-of-Concept Organ-on-Chip Study: Topical Cinnamaldehyde Exposure of Reconstructed Human Skin with Integrated Neopapillae Cultured under Dynamic Flow.Pharmaceutics · 2022Article
- Mesenchymal stem cells' seeded amniotic membrane as a tissue-engineered dressing for wound healing.Drug delivery and translational research · 2022Article
- Systems of conductive skin for power transfer in clinical applications.European biophysics journal : EBJ · 2022Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Autologous split-thickness skin grafts are the preferred treatment for excised burn wounds, but donor sites for autografting are often limited in patients with extensive burns. A number of alternative treatments are already in use to treat large burns and ulcers. Despite intense efforts to develop tissue-engineered skin, delayed or absent vascularization is one of the major reasons for tissue-engineered skin engraftment failure. To overcome these problems, we developed a scaffold-free 3-dimensional (3D) skin substitute containing vascular networks that combine dermal fibroblasts, endothelial cells, and epidermal keratinocytes based on our layer-by-layer cell coating technique. We transplanted the pre-vascularized 3D skin substitutes onto full-thickness skin defects on severe combined immunodeficiency mice to assess their integration with the host tissue and effects on wound healing. We used non-vascularized 3D skin substitutes as a control. Vessels containing red blood cells were evident in the non-vascularized control by day 14. However, blood perfusion of the human-derived vasculature could be detected within 7 days of grafting. Moreover, the pre-vascularized 3D skin substitutes had high graft survival and their epidermal layers were progressively replaced by mouse epidermis. We propose that a novel dermo-epidermal 3D skin substitute containing blood vessels can promote efficient reconstruction of full-thickness skin defects.
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