ArticleProceedings of the National Academy of Sciences of the United States of America2019
Mosaic origin of the eukaryotic kinetochore.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
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Who cites it
74 citing papers in PubMed, 120 citations in OpenAlex.
- Structure and function of the centromere and inner kinetochore.Nature reviews. Molecular cell biology · 2026Review
- Structure and function of the outer kinetochore.Nature reviews. Molecular cell biology · 2026Review
- Systematic identification of germ granule proteins reveals specialized roles in RNAi and small RNA inheritance.Genes & development · 2026Article
- Architecture and function of holocentric CENP-A-independent inner kinetochores.Science advances · 2026Article
- A protein interactome for the last eukaryotic common ancestor illuminates the biochemical basis of modern genetic diseases.Cell genomics · 2026Article
- Assembly and phosphoregulatory mechanisms of the budding yeast outer kinetochore KMN complex.The Journal of cell biology · 2026Article
- Article
- Plasmodium ARK1 regulates spindle formation during atypical mitosis and forms a divergent chromosomal passenger complex.Nature communications · 2026Article
- Kinetochore mutations and histone phosphorylation pattern changes accompany holo- and macro-monocentromere evolution.Nature communications · 2025Article
- Article
- Radiotherapy and precision medicine's role in molecular alterations during chromosomal division: The influence ofBiochemistry and biophysics reports · 2025Article
- The composition and structure of the outer kinetochore KMN complex is conserved across kingdoms.Communications biology · 2025Article
- Eukaryogenesis From FECA to LECA: Radical Steps Along the Way.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025Review
- Plasmodium ARK1 regulates spindle formation during atypical mitosis and forms a divergent chromosomal passenger complex.Research square · 2025Article
- Hypothesis that ancestral eukaryotes sexually proliferated without kinetochores or mitosis.Journal of cell science · 2025Article
- The Spc105/Kre28 complex promotes mitotic error correction by outer kinetochore recruitment of Ipl1/Sli15.The EMBO journal · 2025Article
- Adaptive evolution of CENP-T modulates centromere binding.Current biology : CB · 2025Article
- Review
- An evolutionary perspective on the relationship between kinetochore size and CENP-E dependence for chromosome alignment.Journal of cell science · 2024Article
- Article
14 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The emergence of eukaryotes from ancient prokaryotic lineages embodied a remarkable increase in cellular complexity. While prokaryotes operate simple systems to connect DNA to the segregation machinery during cell division, eukaryotes use a highly complex protein assembly known as the kinetochore. Although conceptually similar, prokaryotic segregation systems and the eukaryotic kinetochore are not homologous. Here we investigate the origins of the kinetochore before the last eukaryotic common ancestor (LECA) using phylogenetic trees, sensitive profile-versus-profile homology detection, and structural comparisons of its protein components. We show that LECA's kinetochore proteins share deep evolutionary histories with proteins involved in a few prokaryotic systems and a multitude of eukaryotic processes, including ubiquitination, transcription, and flagellar and vesicular transport systems. We find that gene duplications played a major role in shaping the kinetochore; more than half of LECA's kinetochore proteins have other kinetochore proteins as closest homologs. Some of these have no detectable homology to any other eukaryotic protein, suggesting that they arose as kinetochore-specific folds before LECA. We propose that the primordial kinetochore evolved from proteins involved in various (pre)eukaryotic systems as well as evolutionarily novel folds, after which a subset duplicated to give rise to the complex kinetochore of LECA.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.