ArticleBiophysical journal2019
Stiffness of MDCK II Cells Depends on Confluency and Cell Size.
Article in Biophysical journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 43 citations in OpenAlex.
- VLA-4 integrin modulates cellular responses to sorafenib in metastasis-related processes of B16F10 melanoma cells.Naunyn-Schmiedeberg's archives of pharmacology · 2026Article
- Integrating 3D Osteocyte Culture, Microgravity Simulation, and Fluid Flow Reveals Mechanisms of Osteocyte Mechanosensation and Calcium Signaling Altered by Disuse.Biomolecules · 2025Article
- Article
- Impact of trypsin on cell cytoplasm during detachment of cells studied by terahertz sensing.Biophysical journal · 2024Article
- A Cell Pre-Wrapping Seeding Technique for Hydrogel-Based Tubular Organ-On-A-Chip.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Size Matters: Rethinking Hertz Model Interpretation for Cell Mechanics Using AFM.International journal of molecular sciences · 2024Article
- Post-translational modifications of beta-amyloid modulate its effect on cell mechanical properties and influence cytoskeletal signaling cascades.Frontiers in molecular neuroscience · 2024Article
- The Effect of Substrate Stiffness on Elastic Force Transmission in the Epithelial Monolayers over Short Timescales.Cellular and molecular bioengineering · 2023Article
- Cingulin and paracingulin tether myosins-2 to junctions to mechanoregulate the plasma membrane.The Journal of cell biology · 2023Article
- Mechanical properties of epithelial cells in domes investigated using atomic force microscopy.Frontiers in cell and developmental biology · 2023Article
- Supracellular measurement of spatially varying mechanical heterogeneities in live monolayers.Biophysical journal · 2022Article
- Thickness of epithelia on wavy substrates: measurements and continuous models.The European physical journal. E, Soft matter · 2022Article
- Two types of critical cell density for mechanical elimination of abnormal cell clusters from epithelial tissue.PLoS computational biology · 2022Article
- Simultaneous stabilization of actin cytoskeleton in multiple nephron-specific cells protects the kidney from diverse injury.Nature communications · 2022Article
- Cancer cell viscoelasticity measurement by quantitative phase and flow stress induction.Biophysical journal · 2022Article
- Tight Junction ZO Proteins Maintain Tissue Fluidity, Ensuring Efficient Collective Cell Migration.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Cell fluorescence photoactivation as a method to select and study cellular subpopulations grown in mechanically heterogeneous environments.Molecular biology of the cell · 2021Article
- Human mammary epithelial cells in a mature, stratified epithelial layer flatten and stiffen compared to single and confluent cells.Biochimica et biophysica acta. General subjects · 2021Article
- Article
- Adhesion of Epithelial Cells to PNIPAm Treated Surfaces for Temperature-Controlled Cell-Sheet Harvesting.ACS applied materials & interfaces · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mechanical phenotyping of adherent cells has become a serious tool in cell biology to understand how cells respond to their environment and eventually to identify disease patterns such as the malignancy of cancer cells. In the steady state, homeostasis is of pivotal importance, and cells strive to maintain their internal stresses even in challenging environments and in response to external chemical and mechanical stimuli. However, a major problem exists in determining mechanical properties because many techniques, such as atomic force microscopy, that assess these properties of adherent cells locally can only address a limited number of cells and provide elastic moduli that vary substantially from cell to cell. The origin of this spread in stiffness values is largely unknown and might limit the significance of measurements. Possible reasons for the disparity are variations in cell shape and size, as well as biological reasons such as the cell cycle or polarization state of the cell. Here, we show that stiffness of adherent epithelial cells rises with increasing projected apical cell area in a nonlinear fashion. This size stiffening not only occurs as a consequence of varying cell-seeding densities, it can also be observed within a small area of a particular cell culture. Experiments with single adherent cells attached to defined areas via microcontact printing show that size stiffening is limited to cells of a confluent monolayer. This leads to the conclusion that cells possibly regulate their size distribution through cortical stress, which is enhanced in larger cells and reduced in smaller cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.