ArticleBMC microbiology2019
Isolation and identification of Brucella melitensis using bacteriological and molecular tools from aborted goats in the Afar region of north-eastern Ethiopia.
Article in BMC microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 34 citations in OpenAlex.
- Brucella outer membrane protein 16 induces trophoblast pyroptosis via rewiring IL-6 trans-signaling and caspase-3/GSDME axis.PLoS neglected tropical diseases · 2026Article
- Prevalence of Brucellosis in Small Ruminants in Africa from 2000 to 2025: A Systematic Review and Meta-Analysis.Veterinary sciences · 2026Review
- Seroprevalence and Associated Factors of Camel and Human Brucellosis in Dire District, Southern Ethiopia: A One-Health Perspective.Veterinary medicine and science · 2026Article
- Sensitivity of current laboratory methods for brucellosis diagnosis at endemic foci.BMC microbiology · 2026Article
- Seroprevalence of camel brucellosis and its relationship to camel owners' knowledge, attitudes, and practices in Dire District, Borana Zone, Ethiopia: A cross-sectional study.One health (Amsterdam, Netherlands) · 2025Article
- Sero-prevalence and associated risk factors of small ruminant's brucellosis in the Yabello and Elweye districts of Borana zone, Southern Ethiopia.BMC veterinary research · 2025Article
- Detection and Molecular Diversity ofPathogens (Basel, Switzerland) · 2024Article
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- Seroprevalence of Brucellosis in Sheep and Goats With Owners' Knowledge, Attitudes and Practices in Garowe District, Nugal region, Somalia.Environmental health insights · 2024Article
- Study on small ruminant brucellosis and owners awareness in two selected districts of southern region, Ethiopia.Veterinary medicine and science · 2023Article
- Whole genome sequencing of EthiopianFrontiers in microbiology · 2023Article
- Isolation and characterisation ofVeterinarni medicina · 2022Article
- Molecular detection of Brucella spp. in ruminant herds in Greece.Tropical animal health and production · 2022Article
- Rapid, ultrasensitive, and highly specific identification ofFrontiers in microbiology · 2022Article
- Sero-prevalence and risk factors of Brucella presence in farm bulk milk from open and zero grazing cattle production systems in Rwanda.Veterinary medicine and science · 2021Article
- Integrated human-animal sero-surveillance of Brucellosis in the pastoral Afar and Somali regions of Ethiopia.PLoS neglected tropical diseases · 2021Article
- Brucellosis: Prevalence with reference to East African community countries - A rapid review.Veterinary medicine and science · 2021Review
- Molecular epidemiology of Brucella species in mixed livestock-human ecosystems in Kenya.Scientific reports · 2021Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundInfection with Brucella melitensis (B. melitensis) is one of the most important causes of abortion in goats and sheep, and also causes severe systemic disease in exposed humans. In Ethiopia, based on seroepidemiological studies, brucellosis is known to be endemic. However, there is little information on the isolation and molecular detection of Brucella species in small ruminants. Therefore, the present study was conducted in the Amibara district of Afar Region of Ethiopia to isolate and molecularly detect Brucella infection in small ruminants.
resultsOut of the total 64 samples cultured, eight samples (five vaginal swabs and three milk) were positive for Brucella species based on colony morphology, growth characteristics, modified acid fast staining and biochemical tests results. Further identification using Brucella- ladder PCR method showed that four of the isolates (three from vaginal swabs and one from milk) from goats amplified fragments of 1071 bp, 794 bp, 587 bp, 450 bp and 152 bp in band size. The molecular result combined with the microbiological and biochemical characteristics of the isolates indicated that the isolates were strains of B. melitensis.
conclusionThe finding of this study could suggest economic and zoonotic significance of B. melitensis and warrants for the need for control strategies in livestock and creation of awareness in the pastoral communities on the safe consumption of foods of animal origin and avoidance of physical contact with aborted materials.
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