Evidence map›Paper›PMID 31125575›Full record

ArticleMolecular and biochemical parasitology2019

The Plasmodium falciparum MESA erythrocyte cytoskeleton-binding (MEC) motif binds to erythrocyte ankyrin.

Geoffrey Kimiti Kilili, Bikash Shakya, Patrick T Dolan, Ling Wang, Monica L Husby, Robert V Stahelin, Ernesto S Nakayasu, Douglas J LaCount

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular and biochemical parasitology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.7field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Identification of ExportedMicroorganisms · 2022
    Article
  3. Article
  4. Sickle Cell Trait Modulates the Proteome and Phosphoproteome ofFrontiers in cellular and infection microbiology · 2021
    Article
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Geoffrey Kimiti KililiDepartment of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907, USA.
Bikash ShakyaDepartment of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907, USA.
Patrick T DolanDepartment of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907, USA.
Ling WangDepartment of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907, USA.
Monica L HusbyDepartment of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907, USA.
Robert V StahelinDepartment of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907, USA.
Ernesto S NakayasuBindley Bioscience Center - Discovery Park, Purdue University, West Lafayette, IN 47907, USA; Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99352, USA.
Douglas J LaCountDepartment of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907, USA. Electronic address: dlacount@purdue.edu.
Purdue University West Lafayette · USPacific Northwest National Laboratory · US

Funding

Indiana Clinical and Translational Sciences InstituteUL1TR001108 · NCATS · INDIANA UNIVERSITY INDIANAPOLIS · PI DENNE, SCOTT C., SHEKHAR, ANANTHA · 2013 to 2017
$23.3M
Emerging virus-host cell protein interaction networksR01AI114814 · NIAID · PURDUE UNIVERSITY · PI DAVEY, ROBERT A, LACOUNT, DOUGLAS J. · 2015 to 2019
$3.5M
A temporal view of the Plasmodium-red blood cell interactomeR01GM092829 · NIGMS · PURDUE UNIVERSITY · PI LACOUNT, DOUGLAS J. · 2010 to 2013
$1.1M
Perturbing virus replication with interfering peptidesR21AI166736 · NIAID · PURDUE UNIVERSITY · PI LACOUNT, DOUGLAS J. · 2022 to 2023
$451k
NCATS NIH HHS UL1 TR001108NIAID NIH HHS R01 AI114814NIAID NIH HHS R21 AI166736NIGMS NIH HHS R01 GM092829
6 · The paper itself

Abstract

The MESA erythrocyte cytoskeleton binding (MEC) motif is a 13-amino acid sequence found in 14 exported Plasmodium falciparum proteins. First identified in the P. falciparum Mature-parasite-infected Erythrocyte Surface Antigen (MESA), the MEC motif is sufficient to target proteins to the infected red blood cell cytoskeleton. To identify host cell targets, purified MESA MEC motif was incubated with a soluble extract from uninfected erythrocytes, precipitated and subjected to mass spectrometry. The most abundant co-purifying protein was erythrocyte ankyrin (ANK1). A direct interaction between the MEC motif and ANK1 was independently verified using co-purification experiments, the split-luciferase assay, and the yeast two-hybrid assay. A systematic mutational analysis of the core MEC motif demonstrated a critical role for the conserved aspartic acid residue at the C-terminus of the MEC motif for binding to both erythrocyte inside-out vesicles and to ANK1. Using a panel of ANK1 constructs, the MEC motif binding site was localized to the ZU5

Indexed as

Amino Acid MotifsAnkyrinsCytoskeletonErythrocytesHumansMalaria, FalciparumPlasmodium falciparumProtein BindingProtozoan ProteinsAnkyrinsMESA protein, Plasmodium falciparumProtozoan ProteinsAnkyrinBand 4.1Exported proteinHost-pathogen interactionMESAPF3D7_0402100PF3D7_0500800Plasmodium

Identifiers

PMID31125575
PMCPMC7020240
OpenAlexW2945566206

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.