ArticleMicrosystems & nanoengineering2019
Separation of spermatozoa from erythrocytes using their tumbling mechanism in a pinch flow fractionation device.
Article in Microsystems & nanoengineering, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 38 citations in OpenAlex.
- Isolation Techniques of Micro/Nano-Scaled Species for Biomedical Applications.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Straining Flow Effects on Sperm Flagellar Energetics in Microfluidic Cross-Slot Traps.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Technologies to improve sperm retrieval in men undergoing micro-TESE for NOA.Asian journal of andrology · 2025Review
- Advancements in differentiation between sperm cells and epithelial cells for efficient forensic DNA analysis in sexual assault cases.International journal of legal medicine · 2024Review
- Microfluidics-A novel technique for high-quality sperm selection for greater ART outcomes.FASEB bioAdvances · 2024Review
- A Review of Research Progress in Microfluidic Bioseparation and Bioassay.Micromachines · 2024Review
- Low-cost inertial microfluidic device for microparticle separation: A laser-Ablated PMMA lab-on-a-chip approach without a cleanroom.HardwareX · 2023Article
- Opportunities involving microfluidics and 3D culture systems to theAnimal reproduction · 2023Review
- Microfluidics geometries involved in effective blood plasma separation.Microfluidics and nanofluidics · 2022Review
- Real-Time Detection of Tumor Cells during Capture on a Filter Element Significantly Enhancing Detection Rate.Biosensors · 2021Article
- Public-Health-Driven Microfluidic Technologies: From Separation to Detection.Micromachines · 2021Review
- An automated instrument for intrauterine insemination sperm preparation.Scientific reports · 2020Article
- Effect of microfluidic processing on the viability of boar and bull spermatozoa.Biomicrofluidics · 2020Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Men suffering from azoospermia can father a child, by extracting spermatozoa from a testicular biopsy sample. The main complication in this procedure is the presence of an abundance of erythrocytes. Currently, the isolation of the few spermatozoa from the sample is manually performed due to ineffectiveness of filtering methods, making it time consuming and labor intensive. The spermatozoa are smaller in both width and height than any other cell type found in the sample, with a very small difference compared with the erythrocyte for the smallest, making this not the feature to base the extraction on. However, the length of the spermatozoon is 5× larger than the diameter of an erythrocyte and can be utilized. Here we propose a microfluidic chip, in which the tumbling behavior of spermatozoa in pinched flow fractionation is utilized to separate them from the erythrocytes. We show that we can extract 95% of the spermatozoa from a sample containing 2.5% spermatozoa, while removing around 90% of the erythrocytes. By adjusting the flow rates, we are able to increase the collection efficiency while slightly sacrificing the purity, tuning the solution for the available sample in the clinic.
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Registered trials
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