ArticleIranian journal of parasitology
A Simple and Cost-Effective Freeze-Thaw Based Method for
Article in Iranian journal of parasitology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Mouse Models for Use in Cryptosporidium Infection Studies, Quantification of Parasite Burden Using Flow Cytometry, qPCR and Histopathology, and Confocal Imaging of Oocysts.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Phenylacetylcarbinol biotransformation by disrupted yeast cells using ultrasonic treatment in conjunction with a dipropylene glycol mediated biphasic emulsion system.Scientific reports · 2025Article
- Revolutionizing DNA Extraction: A Cost-Effective Approach for Genomic DNA Retrieval from Dried Blood Spots.EJIFCC · 2025Article
- Delivery of a sebum modulator by an engineered skin microbe in mice.Nature biotechnology · 2024Article
- Article
- A simple alkali lysis method for Plasmodium falciparum DNA extraction from filter paper blood samples.Molecular and biochemical parasitology · 2023Article
- Development and validation of a flexible DNA extraction (PAN) method for liquid biopsy of multiple sample types.Journal of clinical laboratory analysis · 2021Article
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Authors and funding
7 authors.
Funding
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Abstract
backgroundAvailable DNA isolation methods for
methodsThe protocol involves simple freezing and thawing of DBS, neither involves any purification step nor any chemical reagent. The method was assessed in terms of DNA quantity, PCR detection sensitivity, time requirement, cost effectiveness, labor intensiveness and degree of shearing. The reliability of this method was confirmed by comparing it with other in use methods for
resultsPure DNA was obtained with this method, as exemplified by the absorbance ratio (260nm /280nm) of 1.2. The protocol produced digestible, PCR-grade genomic DNA, also found to be suitable for sequencing. DNA isolated remained stable and retained its integrity after storage for one month at 4 °C.
conclusionOur process substantiated as efficient, reproducible, simple, fast, and inexpensive. Development of this optimized freeze-thaw based DNA extraction method for malaria parasite may provide a valuable tool for molecular analysis in resource-limited setups. This is the first report of DNA extraction from DBS of
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