ArticleScientific reports2019
Latent Infection of Valsa mali in the Seeds, Seedlings and Twigs of Crabapple and Apple Trees is a Potential Inoculum Source of Valsa Canker.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 49 citations in OpenAlex.
- The MdERF1B-miR482c-Horticulture research · 2026Article
- Differences in Valsa Canker Resistance and Its Physiological Mechanisms Among Various Apple Rootstock-Scion Combinations.Plants (Basel, Switzerland) · 2026Article
- Jasmonic Acid Signals Involved inCurrent issues in molecular biology · 2025Article
- Transcriptome sequencing reveals Vmplc1 involved in regulating the pathogenicity of Valsa Mali under low temperature induction.BMC genomics · 2025Article
- Genome-Wide Identification and Analysis of Chitinase GH18 Gene Family inJournal of fungi (Basel, Switzerland) · 2025Article
- Study on Pathogenesis ofJournal of fungi (Basel, Switzerland) · 2025Article
- Apple crown and collar canker and necrosis caused by Cytospora balanejica sp. nov. in Iran.Scientific reports · 2024Article
- Integrated transcriptome and metabolome profiling reveals mechanisms underlying the infection ofFrontiers in microbiology · 2024Article
- Rapid Detection and Quantification of Viable Cells ofMicroorganisms · 2023Article
- Antifungal Activity of 6-Methylcoumarin againstJournal of fungi (Basel, Switzerland) · 2022Article
- Rapid Quantification of InfectiousViruses · 2022Article
- The apple FERONIA receptor-like kinase MdMRLK2 negatively regulates Valsa canker resistance by suppressing defence responses and hypersensitive reaction.Molecular plant pathology · 2022Article
- Microbial diversity composition of apple tree roots and resistance of apple Valsa canker with different grafting rootstock types.BMC microbiology · 2022Article
- An Improved Method for Quantification of ViableMicroorganisms · 2022Article
- Surface-Enhanced Raman Scattering Spectroscopy Combined With Chemical Imaging Analysis for Detecting Apple Valsa Canker at an Early Stage.Frontiers in plant science · 2022Article
- Regulation of phenylpropanoid biosynthesis by MdMYB88 and MdMYB124 contributes to pathogen and drought resistance in apple.Horticulture research · 2020Article
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9 authors at 1 institution in 1 country.
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Abstract
A real-time quantitative PCR assay using a species-specific primer pair was developed to rapidly and accurately quantify Valsa mali, the causative pathogen of apple Valsa canker (AVC), in crabapple seeds, crabapple seedlings, apple twigs and apple seeds. Surveys were conducted in different regions, and crabapple or apple seeds were collected for V. mali detection by qPCR assay. Our results showed that 12.87% to 49.01% of crabapple seeds collected from different regions were positive for V. mali. The exopleura and endopleura were the two major areas of V. mali infection in crabapple seeds. The presence of V. mali infection in crabapple seeds was also confirmed by a high-throughput sequencing approach. With the growth of crabapple seedlings, the concentration of V. mali gDNA in crabapple seedlings gradually increased until eight or more leaf blades emerged. One-year-old twigs from an apple scion nursery were infected with V. mali, and only apple seeds from infected apple trees showing evident Valsa canker symptoms carried V. mali. In conclusion, this study reports that crabapple seeds and apple seeds carried V. mali as latent inoculum sources. V. mali infected not only apple tissues but also crabapple seedlings, which are the rootstocks of apple trees. This study indicated that the inoculum sources for AVC vary. Application of a novel qPCR assay can potentially improve the accuracy of early diagnosis, and is helpful to reveal the epidemic regularity of AVC.
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