ArticleMicrobial cell factories2019
Exploring the xylose paradox in Saccharomyces cerevisiae through in vivo sugar signalomics of targeted deletants.
Article in Microbial cell factories, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Hemoglobin expression reduces aerobic bioconversion of guaiacol in Pseudomonas putida despite non-limiting oxygen.AMB Express · 2026Article
- Article
- Positive selection of efficient ethanol producers from xylose at 45 °C in the yeast Ogataea polymorpha.Scientific reports · 2025Article
- Metabolic engineering of Saccharomyces cerevisiae for efficient utilization of pectin-rich biomass.Journal of microbiology (Seoul, Korea) · 2025Review
- Glucose receptor deletion and engineering: impact on xylose sensing and utilization in Saccharomyces cerevisiae.FEMS yeast research · 2025Article
- Engineering transcriptional regulatory networks for improving second-generation fuel ethanol production inSynthetic and systems biotechnology · 2025Review
- Oxygenation influences xylose fermentation and gene expression in the yeast genera Spathaspora and Scheffersomyces.Biotechnology for biofuels and bioproducts · 2024Article
- Construction of an economical xylose-utilizing Saccharomyces cerevisiae and its ethanol fermentation.FEMS yeast research · 2024Article
- Advances inJournal of fungi (Basel, Switzerland) · 2023Review
- Codon Optimization Improves the Prediction of Xylose Metabolism from Gene Content in Budding Yeasts.Molecular biology and evolution · 2023Article
- Re-evaluation of the impact ofMetabolic engineering communications · 2023Article
- The polyHIS Tract of Yeast AMPK Coordinates Carbon Metabolism with Iron Availability.International journal of molecular sciences · 2023Article
- Using phosphoglucose isomerase-deficient (pgi1Δ) Saccharomyces cerevisiae to map the impact of sugar phosphate levels on D-glucose and D-xylose sensing.Microbial cell factories · 2022Article
- Activation of cryptic xylose metabolism by a transcriptional activator Znf1 boosts up xylitol production in the engineered Saccharomyces cerevisiae lacking xylose suppressor BUD21 gene.Microbial cell factories · 2022Article
- Deletion ofFrontiers in microbiology · 2022Article
- D-Xylose Sensing inInternational journal of molecular sciences · 2021Review
- Exploring metal ion metabolisms to improve xylose fermentation in Saccharomyces cerevisiae.Microbial biotechnology · 2021Article
- Metabolic Changes Induced by Deletion of Transcriptional RegulatorMicroorganisms · 2020Article
- Improving Xylose Fermentation inMicroorganisms · 2020Article
- Simulating Extracellular Glucose Signals Enhances Xylose Metabolism in RecombinantMicroorganisms · 2020Article
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5 authors.
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Abstract
backgroundThere have been many successful strategies to implement xylose metabolism in Saccharomyces cerevisiae, but no effort has so far enabled xylose utilization at rates comparable to that of glucose (the preferred sugar of this yeast). Many studies have pointed towards the engineered yeast not sensing that xylose is a fermentable carbon source despite growing and fermenting on it, which is paradoxical. We have previously used fluorescent biosensor strains to in vivo monitor the sugar signalome in yeast engineered with xylose reductase and xylitol dehydrogenase (XR/XDH) and have established that S. cerevisiae senses high concentrations of xylose with the same signal as low concentration of glucose, which may explain the poor utilization.
resultsIn the present study, we evaluated the effects of three deletions (ira2∆, isu1∆ and hog1∆) that have recently been shown to display epistatic effects on a xylose isomerase (XI) strain. Through aerobic and anaerobic characterization, we showed that the proposed effects in XI strains were for the most part also applicable in the XR/XDH background. The ira2∆isu1∆ double deletion led to strains with the highest specific xylose consumption- and ethanol production rates but also the lowest biomass titre. The signalling response revealed that ira2∆isu1∆ changed the low glucose-signal in the background strain to a simultaneous signalling of high and low glucose, suggesting that engineering of the signalome can improve xylose utilization.
conclusionsThe study was able to correlate the previously proposed beneficial effects of ira2∆, isu1∆ and hog1∆ on S. cerevisiae xylose uptake, with a change in the sugar signalome. This is in line with our previous hypothesis that the key to resolve the xylose paradox lies in the sugar sensing and signalling networks. These results indicate that the future engineering targets for improved xylose utilization should probably be sought not in the metabolic networks, but in the signalling ones.
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