ArticleBMC biology2019
A genetically encoded toolkit of functionalized nanobodies against fluorescent proteins for visualizing and manipulating intracellular signalling.
Article in BMC biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed.
- Article
- Intracellular protein binders for imaging, control and future therapeutics.Nature biomedical engineering · 2026Review
- Mitochondria-lysosome coupling contributes to lysosome acidification and aging.Molecular cell · 2026Article
- Article
- PALB2 and 53BP1 govern post-resection homologous recombination DNA repair.Molecular cell · 2025Article
- Article
- De novo designed Hsp70 activator dissolves intracellular condensates.Cell chemical biology · 2025Article
- Rapid depletion of target proteins in plants by an inducible protein degradation system.The Plant cell · 2024Article
- Detection of transcriptome-wide microRNA-target interactions in single cells with agoTRIBE.Nature biotechnology · 2024Article
- KRAP regulates mitochondrial Ca2+ uptake by licensing IP3 receptor activity and stabilizing ER-mitochondrial junctions.Journal of cell science · 2024Article
- Nuclear lamina strain states revealed by intermolecular force biosensor.Nature communications · 2023Article
- Article
- Research Progresses and Applications of Fluorescent Protein Antibodies: A Review Focusing on Nanobodies.International journal of molecular sciences · 2023Review
- Monitoring extracellular ion and metabolite dynamics with recombinant nanobody-fused biosensors.iScience · 2022Article
- Single-domain near-infrared protein provides a scaffold for antigen-dependent fluorescent nanobodies.Nature methods · 2022Article
- Studying Protein Function Using Nanobodies and Other Protein Binders in Drosophila.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Mandipropamid as a chemical inducer of proximity for in vivo applications.Nature chemical biology · 2022Article
- Structural insights into the binding of nanobodies LaM2 and LaM4 to the red fluorescent protein mCherry.Protein science : a publication of the Protein Society · 2021Article
- Perturbation of kinetochore function using GFP-binding protein in fission yeast.G3 (Bethesda, Md.) · 2021Article
- Protein manipulation using single copies of short peptide tags in cultured cells and inDevelopment (Cambridge, England) · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
backgroundIntrabodies enable targeting of proteins in live cells, but generating specific intrabodies against the thousands of proteins in a proteome poses a challenge. We leverage the widespread availability of fluorescently labelled proteins to visualize and manipulate intracellular signalling pathways in live cells by using nanobodies targeting fluorescent protein tags.
resultsWe generated a toolkit of plasmids encoding nanobodies against red and green fluorescent proteins (RFP and GFP variants), fused to functional modules. These include fluorescent sensors for visualization of Ca
conclusionsThis toolkit greatly expands the utility of intrabodies and will enable a range of approaches for studying and manipulating cell signalling in live cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.