ArticleCell cycle (Georgetown, Tex.)2019
A tension-independent mechanism reduces Aurora B-mediated phosphorylation upon microtubule capture by CENP-E at the kinetochore.
Article in Cell cycle (Georgetown, Tex.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 15 citations in OpenAlex.
- CENP-E initiates chromosome congression by opposing Aurora kinases to promote end-on attachments.Nature communications · 2025Article
- Aurora kinases signaling in cancer: from molecular perception to targeted therapies.Molecular cancer · 2025Review
- Kinesin-7 CENP-E mediates chromosome alignment and spindle assembly checkpoint in meiosis I.Chromosoma · 2024Article
- Kinesin-7 CENP-E in tumorigenesis: Chromosome instability, spindle assembly checkpoint, and applications.Frontiers in molecular biosciences · 2024Review
- Polar Chromosomes-Challenges of a Risky Path.Cells · 2022Review
- Aurora B Tension Sensing Mechanisms in the Kinetochore Ensure Accurate Chromosome Segregation.International journal of molecular sciences · 2021Review
- Physiological relevance of post-translational regulation of the spindle assembly checkpoint protein BubR1.Cell & bioscience · 2021Review
- Kinesin-7 CENP-E regulates the formation and structural maintenance of the acrosome.Cell and tissue research · 2021Article
- Leaving no-one behind: how CENP-E facilitates chromosome alignment.Essays in biochemistry · 2020Review
- Review
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3 authors at 1 institution in 1 country.
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Abstract
During mitosis, Aurora B kinase is required for forming proper bi-oriented kinetochore-microtubule attachments. Current models suggest that tension exerted between a pair of sister-kinetochores (inter-kinetochore stretch) produces a spatial separation of Aurora B kinase from kinetochore-associated microtubule binding substrates, such as the Knl1-Mis12-Ndc80 (KMN) network, resulting in a decrease of phosphorylation and, thus, an increase of affinity for microtubules. Using Single-Molecule High-Resolution Colocalization (SHREC) microscopy analysis of the kinetochore-associated motor CENP-E, we now show that CENP-E undergoes structural rearrangements prior to and after tension generation at the kinetochore, and displays a bi-modal Gaussian distribution on a pair of bi-oriented sister kinetochores. The conformational change of CENP-E depends on its microtubule-stimulated motor motility and the highly flexible coiled-coil between its motor and kinetochore-binding tail domains. Chemical inhibition of the motor motility or perturbations of the coiled-coil domain of CENP-E increases Aurora B-mediated Ndc80 phosphorylation in a tension-independent manner. Metaphase chromosome misalignment caused by CENP-E inhibition can be rescued by chemical inhibition of Aurora B kinase. Furthermore, a pair of monotelic sister-kinetochores shows asymmetric levels of Aurora B-mediated phosphorylation in mono-polar spindles depending on CENP-E motor activity. These results collectively suggest a tension-independent mechanism to reduce Aurora B-mediated phosphorylation of outer kinetochore components in response to microtubule capture by CENP-E.
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