Evidence map›Paper›PMID 31121099›Full record

ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2019

Stereospecific modulation of dimeric rhodopsin.

Tamar Getter, Sahil Gulati, Remy Zimmerman, Yuanyuan Chen, Frans Vinberg, Krzysztof Palczewski

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.4field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 9 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 4 institutions in 1 country.

Tamar GetterDepartment of Ophthalmology, Gavin Herbert Eye Institute, University of California-Irvine, California, USA.
Sahil GulatiDepartment of Ophthalmology, Gavin Herbert Eye Institute, University of California-Irvine, California, USA.
Remy ZimmermanDepartment of Ophthalmology, Gavin Herbert Eye Institute, University of California-Irvine, California, USA.
Yuanyuan ChenDepartment of Ophthalmology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
Frans VinbergDepartment of Ophthalmology and Visual Sciences, University of Utah, Salt Lake City, Utah, USA.
Krzysztof PalczewskiDepartment of Ophthalmology, Gavin Herbert Eye Institute, University of California-Irvine, California, USA.
Case Western Reserve University · USUniversity of California, Irvine · USUniversity of Pittsburgh · USUniversity of Utah · US

Funding

Use of systems pharmacology to prevent rod and cone photoreceptor degenerationR24EY027283 · NEI · UNIVERSITY OF CALIFORNIA-IRVINE · PI PALCZEWSKI, KRZYSZTOF · 2017 to 2021
$7.9M
Assessment of Retinal Function in Health and Disease From Mouse To HumanR00EY026651 · NEI · UNIVERSITY OF UTAH · PI VINBERG, FRANS · 2017 to 2019
$722k
Drug discovery and mechanistic study of P23H rhodopsin associated retinitis pigmentosaR00EY024992 · NEI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI CHEN, YUANYUAN · 2017 to 2019
$711k
Drug discovery and mechanistic study of P23H rhodopsin associated retinitis pigmentosaK99EY024992 · NEI · CASE WESTERN RESERVE UNIVERSITY · PI CHEN, YUANYUAN · 2015 to 2016
$194k
ASSESSMENT OF RETINAL FUNCTION IN HEALTH AND DISEASE FROM MOUSE TO HUMANK99EY026651 · NEI · WASHINGTON UNIVERSITY · PI VINBERG, FRANS · 2016 to 2017
$190k
NEI NIH HHS K99 EY024992NEI NIH HHS K99 EY026651NEI NIH HHS R00 EY024992NEI NIH HHS R00 EY026651NEI NIH HHS R24 EY027283
6 · The paper itself

Abstract

The classic concept that GPCRs function as monomers has been challenged by the emerging evidence of GPCR dimerization and oligomerization. Rhodopsin (Rh) is the only GPCR whose native oligomeric arrangement was revealed by atomic force microscopy demonstrating that Rh exists as a dimer. However, the role of Rh dimerization in retinal physiology is currently unknown. In this study, we identified econazole and sulconazole, two small molecules that disrupt Rh dimer contacts, by implementing a cell-based high-throughput screening assay. Racemic mixtures of identified lead compounds were separated and tested for their stereospecific binding to Rh using UV-visible spectroscopy and intrinsic fluorescence of tryptophan (Trp) 265 after illumination. By following the changes in UV-visible spectra and Trp265 fluorescence

Indexed as

Cell Line, TumorCell SurvivalEconazoleElectrophysiologyHumansImidazolesImmunoblottingKineticsProtein MultimerizationRhodopsinEconazoleImidazolesRhodopsinsulconazolechromophoreGPCR dimerizationG-protein-coupled receptorshigh-throughput screeningisomerization

Identifiers

PMID31121099
PMCPMC6662988
OpenAlexW2944995184

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.