ArticlePloS one2019
Physical events occurring during the cryopreservation of immortalized human T cells.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 44 citations in OpenAlex.
- Raman spectroscopic analysis of intracellular ice-induced degradation of mesenchymal stromal cells.Biophysical journal · 2026Article
- Basic Points to Consider for Cell Storage under the Act on the Safety of Regenerative Medicine in Japan.Regenerative therapy · 2025Review
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- Cold Chain Delivery in Cell Therapies.Advances in experimental medicine and biology · 2025Review
- Barriers to Effective Cryopreservation of Cell Therapies: Challenges and Emerging Solution.Advances in experimental medicine and biology · 2025Review
- Article
- Successful expansion and cryopreservation of human natural killer cell line NK-92 for clinical manufacturing.PloS one · 2024Article
- The impact of repeated temperature cycling on cryopreserved human iPSC viability stems from cytochrome redox state changes.Frontiers in bioengineering and biotechnology · 2024Article
- Cryopreservation of brain cell structure: a review.Free neuropathology · 2024Article
- Sound waves for solving the problem of recrystallization in cryopreservation.Scientific reports · 2023Article
- Improving Cell Recovery: Freezing and Thawing Optimization of Induced Pluripotent Stem Cells.Cells · 2022Review
- On crystallization of water confined in liposomes and cryoprotective action of DMSO.RSC advances · 2022Article
- Natural deep eutectic systems for nature-inspired cryopreservation of cells.AIChE journal. American Institute of Chemical Engineers · 2021Article
- Biomolecular Perturbations in In-Cell Dynamic Nuclear Polarization Experiments.Frontiers in molecular biosciences · 2021Article
- The transfer temperature from slow cooling to cryogenic storage is critical for optimal recovery of cryopreserved mammalian cells.PloS one · 2021Article
- Cryopreservation of primary cultures of mammalian somatic cells in 96-well plates benefits from control of ice nucleation.Cryobiology · 2020Article
- Cryopreservation as a Key Element in the Successful Delivery of Cell-Based Therapies-A Review.Frontiers in medicine · 2020Review
- Cryopreservation of Human Midbrain Dopaminergic Neural Progenitor Cells Poised for Neuronal Differentiation.Frontiers in cell and developmental biology · 2020Article
- Automated dry thawing of cryopreserved haematopoietic cells is not adversely influenced by cryostorage time, patient age or gender.PloS one · 2020Article
- Optimizing cryopreservation strategies for scalable cell therapies: A comprehensive review with insights from iPSC-derived therapies.Biotechnology progressReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cryopreservation is key for delivery of cellular therapies, however the key physical and biological events during cryopreservation are poorly understood. This study explored the entire cooling range, from membrane phase transitions above 0°C to the extracellular glass transition at -123°C, including an endothermic event occurring at -47°C that we attributed to the glass transition of the intracellular compartment. An immortalised, human suspension cell line (Jurkat) was studied, using the cryoprotectant dimethyl sulfoxide. Fourier transform infrared spectroscopy was used to determine membrane phase transitions and differential scanning calorimetry to analyse glass transition events. Jurkat cells were exposed to controlled cooling followed by rapid, uncontrolled cooling to examine biological implications of the events, with post-thaw viable cell number and functionality assessed up to 72 h post-thaw. The intracellular glass transition observed at -47°C corresponded to a sharp discontinuity in biological recovery following rapid cooling. No other physical events were seen which could be related to post-thaw viability or performance significantly. Controlled cooling to at least -47°C during the cryopreservation of Jurkat cells, in the presence of dimethyl sulfoxide, will ensure an optimal post-thaw viability. Below -47°C, rapid cooling can be used. This provides an enhanced physical and biological understanding of the key events during cryopreservation and should accelerate the development of optimised cryobiological cooling protocols.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.