ArticleACS applied materials & interfaces2019
Mechanically Biomimetic Gelatin-Gellan Gum Hydrogels for 3D Culture of Beating Human Cardiomyocytes.
Article in ACS applied materials & interfaces, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.
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Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Advances in Engineering Human Tissue Models.Frontiers in bioengineering and biotechnology · 2020Pooled it
- Mechanical and Electrical Phenotype of hiPSC-Cardiomyocytes on Fibronectin-Based Hydrogels.Advanced healthcare materials · 2026Article
- Balancing Strength and Cell Viability in Gelatin Methacrylate/Gellan Gum Bioink Formulations.ACS omega · 2026Article
- Quantitative Assessment of Hydrogel Printability in Extrusion Bioprinting.Gels (Basel, Switzerland) · 2026Review
- Decellularized Extracellular Matrix/Gellan Gum Hydrogels Enriched with Spermine for Cardiac Models.Gels (Basel, Switzerland) · 2026Article
- 3D Printing for Tissue Engineering: Printing Techniques, Biomaterials, Challenges, and the Emerging Role of 4D Bioprinting.Bioengineering (Basel, Switzerland) · 2025Review
- Scaffold Biomaterials in the Development of Cultured Meat: A Review.Food science of animal resources · 2025Review
- Gellan gum-gelatin based cardiac models support formation of cellular networks and functional cardiomyocytes.Cytotechnology · 2024Article
- The Need for Research on the Comparison of Sensory Characteristics between Cultured Meat Produced Using Scaffolds and Meat.Food science of animal resources · 2024Review
- Three-Dimensionally Printed Hydrogel Cardiac Patch for Infarct Regeneration Based on Natural Polysaccharides.Polymers · 2023Article
- Therapeutic angiogenesis and tissue revascularization in ischemic vascular disease.Journal of biological engineering · 2023Review
- Fluorescent hiPSC-derived MYH6-mScarlet cardiomyocytes for real-time tracking, imaging, and cardiotoxicity assays.Cell biology and toxicology · 2023Article
- Quartz Crystal Microbalance Technology Coupled with Impedance for the Dynamic Monitoring of the Cardiomyocyte Beating Function and Drug Screening.Biosensors · 2023Article
- Building blocks of microphysiological system to model physiology and pathophysiology of human heart.Frontiers in physiology · 2023Review
- Article
- Hydrogel supplemented with human platelet lysate enhances multi-lineage differentiation of mesenchymal stem cells.Journal of nanobiotechnology · 2022Article
- Addition of High Acyl Gellan Gum to Low Acyl Gellan Gum Enables the Blends 3D Bioprintable.Gels (Basel, Switzerland) · 2022Article
- Valuable effect of Manuka Honey in increasing the printability and chondrogenic potential of a naturally derived bioink.Materials today. Bio · 2022Article
- Effect of hydroxychloroquine sulfate on the gelation behavior, water mobility and structure of gelatin.Colloids and surfaces. A, Physicochemical and engineering aspects · 2022Article
- A cell adhesion-promoting multi-network 3D printing bio-ink based on natural polysaccharide hydrogel.Frontiers in bioengineering and biotechnology · 2022Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To promote the transition of cell cultures from 2D to 3D, hydrogels are needed to biomimic the extracellular matrix (ECM). One potential material for this purpose is gellan gum (GG), a biocompatible and mechanically tunable hydrogel. However, GG alone does not provide attachment sites for cells to thrive in 3D. One option for biofunctionalization is the introduction of gelatin, a derivative of the abundant ECM protein collagen. Unfortunately, gelatin lacks cross-linking moieties, making the production of self-standing hydrogels difficult under physiological conditions. Here, we explore the functionalization of GG with gelatin at biologically relevant concentrations using semiorthogonal, cytocompatible, and facile chemistry based on hydrazone reaction. These hydrogels exhibit mechanical behavior, especially elasticity, which resembles the cardiac tissue. The use of optical projection tomography for 3D cell microscopy demonstrates good cytocompatibility and elongation of human fibroblasts (WI-38). In addition, human-induced pluripotent stem cell-derived cardiomyocytes attach to the hydrogels and recover their spontaneous beating in 24 h culture. Beating is studied using in-house-built phase contrast video analysis software, and it is comparable with the beating of control cardiomyocytes under regular culture conditions. These hydrogels provide a promising platform to transition cardiac tissue engineering and disease modeling from 2D to 3D.
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