ArticleThe FEBS journal2019
Phosphorylation of Arabidopsis eIF4E and eIFiso4E by SnRK1 inhibits translation.
Article in The FEBS journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 36 citations in OpenAlex.
- Pioneering plant virology research for a healthier future: a summary of the 2025 American Society for Virology (ASV) Plant Virology Satellite Symposium.Journal of virology · 2026Article
- Proteome-Transcriptome Discordance in Rice Under Drought Is Modulated by Post-Translational Modifications with Functional Consequences for Photosynthesis and Energy Metabolism.Plants (Basel, Switzerland) · 2026Article
- Translational reprogramming under heat stress: a plant's perspective.Royal Society open science · 2025Review
- Modern Plant Breeding Techniques in Crop Improvement and Genetic Diversity: From Molecular Markers and Gene Editing to Artificial Intelligence-A Critical Review.Plants (Basel, Switzerland) · 2024Review
- What, where, and how: Regulation of translation and the translational landscape in plants.The Plant cell · 2024Review
- Review
- Structure of the actively translating plant 80S ribosome at 2.2 Å resolution.Nature plants · 2023Article
- Review
- Plant translational reprogramming for stress resilience.Frontiers in plant science · 2023Review
- Mapping of the plant SnRK1 kinase signalling network reveals a key regulatory role for the class II T6P synthase-like proteins.Nature plants · 2022Article
- SNF1-Related Protein Kinase 1 Activity Represses the Canonical Translational Machinery.Plants (Basel, Switzerland) · 2022Article
- Dynamic Nutrient Signaling Networks in Plants.Annual review of cell and developmental biology · 2021Review
- Phosphorylation of a reinitiation supporting protein, RISP, determines its function in translation reinitiation.Nucleic acids research · 2021Article
- Translational gene regulation in plants: A green new deal.Wiley interdisciplinary reviews. RNA · 2020Review
- Characterization of Local and Systemic Impact of Whitefly (International journal of molecular sciences · 2020Article
- Abscisic Acid Connects Phytohormone Signaling with RNA Metabolic Pathways and Promotes an Antiviral Response that Is Evaded by a Self-Controlled RNA Virus.Plant communications · 2020Article
- Evidence That Phosphorylation of theFrontiers in plant science · 2020Article
- Manipulation of the Plant Host by the Geminivirus AC2/C2 Protein, a Central Player in the Infection Cycle.Frontiers in plant science · 2020Review
- A Novel Interaction Network Used by Potyviruses in Virus-Host Interactions at the Protein Level.Viruses · 2019Article
- A new mechanism for translational control in plants.The FEBS journal · 2019Article
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4 authors at 2 institutions in 1 country.
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Abstract
Regulation of protein synthesis is critical for maintaining cellular homeostasis. In mammalian systems, translational regulatory networks have been elucidated in considerable detail. In plants, however, regulation occurs through different mechanisms that remain largely elusive. In this study, we present evidence that the Arabidopsis thaliana energy sensing kinase SnRK1, a homologue of mammalian AMP-activated kinase and yeast sucrose non-fermenting 1 (SNF1), inhibits translation by phosphorylating the cap binding proteins eIF4E and eIFiso4E. We establish that eIF4E and eIFiso4E contain two deeply conserved SnRK1 consensus target sites and that both interact with SnRK1 in vivo. We then demonstrate that SnRK1 phosphorylation inhibits the ability of Arabidopsis eIF4E and eIFiso4E to complement a yeast strain lacking endogenous eIF4E, and that inhibition correlates with repression of polysome formation. Finally, we show that SnRK1 over-expression in Nicotiana benthamiana plants reduces polysome formation, and that this effect can be counteracted by transient expression of eIF4E or mutant eIF4E containing non-phosphorylatable SnRK1 target residues, but not by a phosphomimic eIF4E. Together, these studies elucidate a novel and direct pathway for translational control in plant cells. In light of previous findings that SnRK1 conditions an innate antiviral defense and is inhibited by geminivirus pathogenicity factors, we speculate that phosphorylation of cap binding proteins may be a component of the resistance mechanism.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.