Evidence map›Paper›PMID 31119714›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2020

Generation of a Full-Thickness Human Skin Equivalent on an Immunodeficient Mouse.

Nicole Diette, Igor Kogut, Ganna Bilousova

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Nicole DietteDepartment of Dermatology, University of Colorado School of Medicine, Anschutz Medical Campus, Aurora, CO, USA.
Igor KogutDepartment of Dermatology, University of Colorado School of Medicine, Anschutz Medical Campus, Aurora, CO, USA.
Ganna BilousovaDepartment of Dermatology, University of Colorado School of Medicine, Anschutz Medical Campus, Aurora, CO, USA. Ganna.Bilousova@UCDenver.edu.
University of Colorado Anschutz Medical Campus · US

Funding

Transgenic and Gene Targeting CoreP30AR057212 · NIAMS · UNIVERSITY OF COLORADO DENVER · PI NORRIS, DAVID A. · 2009 to 2018
$6.2M
Training in Translational Research of Skin DiseasesT32AR007411 · NIAMS · UNIVERSITY OF COLORADO DENVER · PI ASGARI, MARYAM MANDANA, ROOP, DENNIS · 1986 to 2025
$4.8M
MicroRNA-based purification of keratinocytes derived from pluripotent stem cells for the treatment of skin diseasesR21AR074642 · NIAMS · UNIVERSITY OF COLORADO DENVER · PI BILOUSOVA, GANNA · 2019 to 2020
$376k
NIAMS NIH HHS P30 AR057212NIAMS NIH HHS R21 AR074642NIAMS NIH HHS T32 AR007411
6 · The paper itself

Abstract

Human skin equivalents composed of epidermal cells and fibroblasts are important for modeling human epidermal development, testing new therapeutics, and designing novel treatment strategies for human skin diseases. Here, we describe a procedure for the generation of an in vivo full-thickness human skin equivalent on an immunodeficient mouse using a grafting chamber system. The protocol involves mixing human epidermal cells and fibroblasts in a silicone grafting chamber that is surgically inserted onto the muscle fascia of a recipient immunodeficient mouse. Following the removal of the silicone chamber, the graft area is exposed to air to induce stratification of developing epidermis, resulting in the reconstitution of full-thickness human skin tissue on a live mouse. This grafting system provides a straightforward approach to study human skin diseases in an animal model and has been previously used to determine the ability of both mouse and human primary epidermal cells and cells derived from pluripotent stem cells to regenerate functional skin in vivo.

Indexed as

AnimalsCells, CulturedFibroblastsHumansImmunocompromised HostKeratinocytesLiverMiceMice, Inbred NODModels, AnimalPrimary Cell CultureSkin TransplantationTissue Culture TechniquesEpidermal cellsFibroblastsGrafting chamberHuman skin graftKeratinocytesMouse modelXenograft

Identifiers

PMID31119714
PMCPMC6868296
OpenAlexW3001832222

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.