Evidence map›Paper›PMID 31119627›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2019

Generation of Metal-Depleted Conditions for In Vitro Growth of Neisseria gonorrhoeae.

Cynthia Nau Cornelissen

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

1 author.

Cynthia Nau CornelissenDepartment of Microbiology and Immunology, School of Medicine, Virginia Commonwealth University, Richmond, VA, USA. cynthia.cornelissen@vcuhealth.org.

Funding

Using gonococcal TonB-dependent transporters as vaccine antigensU19AI144182 · NIAID · GEORGIA STATE UNIVERSITY · PI CORNELISSEN, CYNTHIA N · 2019 to 2023
$9.3M
Neisseria gonorrhoeae metal transporters that subvert nutritional immunityR01AI127793 · NIAID · VIRGINIA COMMONWEALTH UNIVERSITY · PI CYNTHIA N CORNELISSEN, Alison K Criss · 2017 to 2026
$6.8M
Rational design of transferrin binding protein-based vaccines to combat gonorrheaR01AI125421 · NIAID · VIRGINIA COMMONWEALTH UNIVERSITY · PI CORNELISSEN, CYNTHIA N, GRAY-OWEN, SCOTT D · 2017 to 2021
$3.1M
NIAID NIH HHS R01 AI125421NIAID NIH HHS R01 AI127793NIAID NIH HHS U19 AI144182
6 · The paper itself

Abstract

Neisseria gonorrhoeae employs high-affinity metal acquisition systems to obtain necessary nutrients, such as iron (Fe) and zinc (Zn) from the environment. Because growth and replication depend upon successful metal acquisition, these high-affinity uptake systems are important virulence factors. Expression of metal acquisition systems is tightly controlled and preferentially expressed under low-metal conditions. Therefore, in order to optimally produce these transport proteins and study them in vitro, growth media must be deployed that mimic low-metal conditions. This chapter describes the chelators, media, and culturing conditions that can generate low-metal in vitro growth conditions.

Indexed as

Bacterial ProteinsCarrier ProteinsCell Culture TechniquesChelating AgentsCulture MediaIronNeisseria gonorrhoeaeVirulence FactorsZincBacterial ProteinsCarrier ProteinsChelating AgentsCulture MediaIronVirulence FactorsZincCalprotectinChelatorIronNeisseria gonorrhoeaeTransferrinZinc

Identifiers

PMID31119627
PMCPMC7375410

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.