ArticleThe Journal of reproduction and development2019
Effect of trehalose on the preservation of freeze-dried mice spermatozoa at room temperature.
Article in The Journal of reproduction and development, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 30 citations in OpenAlex.
- Comparative Sensitivity of Canine Swim-Up Sperm Fractions Treated with Antifreeze Protein and Trehalose Following Freeze-Thaw.Animals : an open access journal from MDPI · 2026Article
- Stallion Sperm Lyophilisation: Effect of Source of Sperm and Reproductive Season.Veterinary sciences · 2026Article
- Monosodium glutamate protects DNA of freeze-dried somatic cells and improves development of somatic cell nuclear transfer embryos.The Journal of reproduction and development · 2026Article
- The challenges and prospects of mammalian reproduction in extraterrestrial environments.The Journal of reproduction and development · 2026Review
- Freeze-Drying and Room Temperature Preservation of Mammalian Sperm.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Improved birth rates via rehydration of mouse freeze-dried spermatozoa using high-temperature ultrapure water.PloS one · 2026Article
- Monosodium glutamate enhances freeze-dried mouse sperm quality during room-temperature storage.Scientific reports · 2025Article
- Can Humanity Thrive Beyond the Galaxy?The Journal of reproduction and development · 2025Review
- Method for long-term room temperature storage of mouse freeze-dried sperm.Scientific reports · 2025Article
- Production of Healthy Mice from Freeze-Dried Spermatozoa Preserved at Room Temperature.Methods in molecular biology (Clifton, N.J.) · 2025Article
- A novel, simplified method to prepare and preserve freeze-dried mouse sperm in plastic microtubes.The Journal of reproduction and development · 2023Article
- Production of mouse offspring from zygotes fertilized with freeze-dried spermatids.Scientific reports · 2022Article
- Production of offspring from vacuum-dried mouse spermatozoa and assessing the effect of drying conditions on sperm DNA and embryo development.The Journal of reproduction and development · 2022Article
- Mouse in vivo-derived late 2-cell embryos have higher developmental competence after high osmolality vitrification and -80°C preservation than IVF or ICSI embryos.The Journal of reproduction and development · 2022Article
- Long-term preservation of germ cells and gonadal tissues at ambient temperatures.Reproduction & fertility · 2022Review
- Desiccated cat spermatozoa retain DNA integrity and developmental potential after prolonged storage and shipping at non-cryogenic temperatures.Journal of assisted reproduction and genetics · 2022Article
- Protocol to preserve mouse freeze-dried spermatozoa in the thin plastic sheets.STAR protocols · 2021Article
- Mailing viable mouse freeze-dried spermatozoa on postcards.iScience · 2021Article
- Evaluating the long-term effect of space radiation on the reproductive normality of mammalian sperm preserved on the International Space Station.Science advances · 2021Article
- Improvement of a twice collection method of mouse oocytes by surgical operation.The Journal of reproduction and development · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Freeze-drying of spermatozoa is a convenient and safe method to preserve mammalian genetic material without the use of liquid nitrogen or a deep freezer. However, freeze-dried spermatozoa (FD sperm) are not frequently used because of the low success rate of offspring after intracytoplasmic spermatozoa injection (ICSI). In this study, we determined the optimal concentration and a point of action of trehalose as a protectant for the preservation of FD sperm from different mouse strains at room temperature (RT). Although trehalose demonstrated no potential to protect the FD sperm of ICR mice against the freeze-drying procedure itself, the blastocyst rate was significantly improved when FD sperm was preserved for more than 1 month at RT (56-63% vs. 29% without trehalose). The optimal concentration of trehalose was 0.5 M. Importantly, remarkable results were obtained when spermatozoa of inbred mouse strains (C57BL/6N, C3H/He, and 129/Sv) were used, and many offspring were obtained when FD sperm that was preserved for 3 months at RT (26-28% vs. 6-11% of without trehalose) was used. However, when DNA damage in FD sperm was examined by gamma-H2Ax assays, it was found that trehalose failed to protect the FD sperm from DNA damage. These results suggest that trehalose has the potential to protect other sperm factors rather than sperm DNA during preservation at RT for longer periods and trehalose is more effective for inbred mouse strains.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.