ArticleJournal of orthopaedic surgery and research2019
Concentrated growth factor promotes proliferation, osteogenic differentiation, and angiogenic potential of rabbit periosteum-derived cells in vitro.
Article in Journal of orthopaedic surgery and research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 2 syntheses or guidelines pooled it, 37 citations in OpenAlex.
- Effectiveness of concentrated growth factors with or without grafting materials in maxillary sinus augmentation: a systematic review.BMC oral health · 2024Pooled it
- Pooled it
- Prospective, randomized study on the effects of autologous concentrated growth factors in the treatment of cystic lesions of the jaw.Wiener klinische Wochenschrift · 2026Trial
- Volumetric evaluation of effects of platelet-rich fibrin and concentrated growth factor on early bone healing after endodontic microsurgery: a randomized controlled trial.BMC oral health · 2023Trial
- Concentrated growth factor in oral bone regeneration: A narrative review.Regenerative therapy · 2026Review
- Comparative Efficacy of Intra-Articular Growth Factor Concentrate and Platelet-Rich Plasma Injections in Knee Osteoarthritis: An Evaluation of Clinical, Radiological, and Biomarker Outcomes.Journal of orthopaedic case reports · 2026Article
- The use of concentrated growth factors in guiding bone regeneration after microsurgical endodontic surgery for periapical lesions.Regenerative biomaterials · 2025Article
- Transcriptome Analysis of Platelet-Rich Plasma-Treated Osteoarthritic Chondrocyte.BioMed research international · 2024Article
- Characterization and potential of periosteum-derived cells: an overview.Frontiers in medicine · 2023Review
- Use of CGF in Oral and Implant Surgery: From Laboratory Evidence to Clinical Evaluation.International journal of molecular sciences · 2022Article
- Innovative Concepts and Recent Breakthrough for Engineered Graft and Constructs for Bone Regeneration: A Literature Systematic Review.Materials (Basel, Switzerland) · 2022Review
- Concentrated Growth Factors Promote hBMSCs Osteogenic Differentiation in a Co-Culture System With HUVECs.Frontiers in bioengineering and biotechnology · 2022Article
- Concentrated Growth Factors Combined with Lipopolysaccharide Stimulate the In Vitro Regenerative and Osteogenic Activities of Human Dental Pulp Stem Cells by Balancing Inflammation.International journal of dentistry · 2022Article
- Analysis of CGF Biomolecules, Structure and Cell Population: Characterization of the Stemness Features of CGF Cells and Osteogenic Potential.International journal of molecular sciences · 2021Article
- Angiogenic Properties of Concentrated Growth Factors (CGFs): The Role of Soluble Factors and Cellular Components.Pharmaceutics · 2021Article
- Biomaterial-based strategies for maxillofacial tumour therapy and bone defect regeneration.International journal of oral science · 2021Review
- Potential of Lyophilized Platelet Concentrates for Craniofacial Tissue Regenerative Therapies.Molecules (Basel, Switzerland) · 2021Review
- Concentrated Growth Factors (CGF) Induce Osteogenic Differentiation in Human Bone Marrow Stem Cells.Biology · 2020Article
- Therapeutic effect of concentrated growth factor preparation on skin photoaging in a mouse model.The Journal of international medical research · 2020Article
- Immobilization of BMP-2 and VEGF within Multilayered Polydopamine-Coated Scaffolds and the Resulting Osteogenic and Angiogenic Synergy of Co-Cultured Human Mesenchymal Stem Cells and Human Endothelial Progenitor Cells.International journal of molecular sciences · 2020Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
objectiveThe aim of this research is to investigate the effects of concentrated growth factor (CGF) on the proliferation, osteogenic differentiation, and angiogenic potential of rabbit periosteum-derived cells (PDCs) in vitro.
methodsPDCs were isolated from the femoral and tibial periosteum of rabbits and cultured with or without CGF membranes or CGF conditioned media. Scanning electron microscopy (SEM) was used for the structural characterization. Cell Counting Kit-8 assay was used to measure cell proliferation. Alkaline phosphatase (ALP) activity of PDCs was also measured. Immunohistochemistry was used to detect the expression of CD34. Enzyme-linked immunosorbent assay (ELISA), quantitative real-time PCR (qPCR), and Western blot were used to evaluate the secretion and expression levels of osteogenic differentiation markers (bone morphogenetic protein-2, type I collagen, osteocalcin) and angiogenesis markers (vascular endothelial growth factor, basic fibroblast growth factor) in supernatants and PDCs at days 3, 7, 14, and 21.
resultsThe SEM analysis showed a dense three-dimensional fibrin network in CGF, and CGF membranes were covered by PDCs with elongated or polygonal morphological features. Compared with the control group, CGF significantly promoted the proliferation of PDCs during the experimental period (p < 0.05). Immunohistochemistry revealed that PDCs were dispersedly distributed among the CGF substrates, and CD34-positive cells were also present. Moreover, CGF significantly increased the ALP activity and upregulated the expression and secretion of osteogenic differentiation and angiogenesis markers in PDCs at days 3, 7, 14, and 21 (p < 0.05).
conclusionCGF can increase the proliferation and promote the osteogenic differentiation and angiogenic potential of PDCs in vitro. These results indicate that CGF can be used as a new therapeutic means for biotechnological and clinical applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.