ArticleACS chemical biology2019
Depsipeptide Aspergillicins Revealed by Chromatin Reader Protein Deletion.
Article in ACS chemical biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 39 citations in OpenAlex.
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- SntB triggers the antioxidant pathways to regulate development and aflatoxin biosynthesis ineLife · 2024Article
- An environmental isolate ofFrontiers in microbiology · 2024Article
- The histone demethylase KdmB is part of a trimeric protein complex and mediates virulence and mycotoxin production in Penicillium expansum.Fungal genetics and biology : FG & B · 2023Article
- Mining theJournal of fungi (Basel, Switzerland) · 2023Article
- Depsipeptides Targeting Tumor Cells: Milestones from In Vitro to Clinical Trials.Molecules (Basel, Switzerland) · 2023Review
- Identifying Fungal Secondary Metabolites and Their Role in Plant Pathogenesis.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Genetic Regulation of Mycotoxin Biosynthesis.Journal of fungi (Basel, Switzerland) · 2022Review
- Macrophage-targeting oligopeptides fromChemical science · 2022Article
- Post-translational modifications drive secondary metabolite biosynthesis in Aspergillus: a review.Environmental microbiology · 2022Review
- A methanolic extract of Zanthoxylum bungeanum modulates secondary metabolism regulator genes in Aspergillus flavus and shuts down aflatoxin production.Scientific reports · 2022Article
- Article
- Microevolution in the pansecondary metabolome ofProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Fungal oxylipins direct programmed developmental switches in filamentous fungi.Nature communications · 2020Article
- The tetrameric pheromone module SteC-MkkB-MpkB-SteD regulates asexual sporulation, sclerotia formation and aflatoxin production in Aspergillus flavus.Cellular microbiology · 2020Article
- Article
- New Insight Into Pathogenicity and Secondary Metabolism of the Plant PathogenFrontiers in microbiology · 2020Article
- The Biogenetic Origin of the Biologically Active Naematolin of Hypholoma Species Involves an Unusual Sesquiterpene Synthase.Molecular biotechnology · 2019Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Expression of biosynthetic gene clusters (BGCs) in filamentous fungi is highly regulated by epigenetic remodeling of chromatin structure. Two classes of histone modifying proteins, writers (which place modifications on histone tails) and erasers (which remove the modifications), have been used extensively to activate cryptic BGCs in fungi. Here, for the first time, we present activation of a cryptic BGC by a third category of histone modifying proteins, reader proteins that recognize histone tail modifications and commonly mediate writer and eraser activity. Loss of the reader SntB (Δ sntB) resulted in the synthesis of two cryptic cyclic hexa-depsipeptides, aspergillicin A and aspergillicin F, in the fungus Aspergillus flavus. Liquid chromatography, high resolution mass spectrometry, and NMR analysis coupled with bioinformatic analysis and gene deletion experiments revealed that a six adenylation (A) domain nonribosomal peptide synthetase (NRPS, called AgiA) and O-methyltransferase (AgiB) were required for metabolite formation. A proposed biosynthetic scheme illustrates the requirement for unusual NRPS domains, such as a starting condensation domain and a thiolesterase domain proposed to cyclize the depsipeptides. This latter activity has only been found in bacterial but not fungal NRPS. The agi BGC-unique to A. flavus and some closely related species (e.g., A. oryzae, A. arachidicola)-is located next to a conserved Aspergillus siderophore BGC syntenic to other fungi.
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