Evidence map›Paper›PMID 31117243›Full record

ArticleMolecules (Basel, Switzerland)2019

Computational Screening and Analysis of Lung Cancer Related Non-Synonymous Single Nucleotide Polymorphisms on the Human Kirsten Rat Sarcoma Gene.

Qiankun Wang, Aamir Mehmood, Heng Wang, Qin Xu, Yi Xiong, Dong-Qing Wei

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecules (Basel, Switzerland), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
1.8field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 40 citations in OpenAlex.

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  11. BC-TFdb: a database of transcription factor drivers in breast cancer.Database : the journal of biological databases and curation · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Qiankun WangState Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China. wangqiankun@sjtu.edu.cn.
Aamir MehmoodState Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China. aamirmehmood@sjtu.edu.cn.ORCID 0000-0001-8713-966X
Heng WangState Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China. wangheng0802@sjtu.edu.cn.
Qin XuState Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China. xuqin523@sjtu.edu.cn.
Yi XiongState Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China. xiongyi@sjtu.edu.cn.ORCID 0000-0003-2910-6725
Dong-Qing WeiState Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China. dqwei@sjtu.edu.cn.ORCID 0000-0003-4200-7502
Shanghai Jiao Tong University · CN

Funding

National Key Research Program 2016YFA0501703National Natural Science Foundation of China 31601074, 31770772, 61872094, 61832019School of Medicine, Shanghai Jiao Tong University YG2017ZD14
6 · The paper itself

Abstract

The human KRAS (Kirsten rat sarcoma) is an oncogene, involved in the regulation of cell growth and division. The mutations in the KRAS gene have the potential to cause normal cells to become cancerous in human lungs. In the present study, we focus on non-synonymous single nucleotide polymorphisms (nsSNPs), which are point mutations in the DNA sequence leading to the amino acid variants in the encoded protein. To begin with, we developed a pipeline to utilize a set of computational tools in order to obtain the most deleterious nsSNPs (Q22K, Q61P, and Q61R) associated with lung cancer in the human KRAS gene. Furthermore, molecular dynamics simulation and structural analyses of the 3D structures of native and mutant proteins confirmed the impact of these nsSNPs on the stability of the protein. Finally, the experimental results demonstrated that the structural stability of the mutant proteins was worse than that of the native protein. This study provides significant guidance for narrowing down the number of KRAS mutations to be screened as potential diagnostic biomarkers and to better understand the structural and functional mechanisms of the KRAS protein.

Indexed as

Amino Acid SubstitutionBiomarkers, TumorComputational BiologyHumansLung NeoplasmsMolecular ConformationMolecular Dynamics SimulationMutationPolymorphism, Single NucleotideProto-Oncogene Proteins p21(ras)Structure-Activity RelationshipBiomarkers, TumorKRAS protein, humanProto-Oncogene Proteins p21(ras)functional effectmolecular dynamics simulationmutationsingle nucleotide polymorphismstructural analysis

Identifiers

PMID31117243
PMCPMC6572712
OpenAlexW2945409063

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.