Evidence map›Paper›PMID 31116972›Full record

ArticleCell reports2019

Transient, Consequential Increases in Extracellular Potassium Ions Accompany Channelrhodopsin2 Excitation.

J Christopher Octeau, Mohitkumar R Gangwani, Sushmita L Allam, Duy Tran, Shuhan Huang, Tuan M Hoang-Trong, Peyman Golshani, Timothy H Rumbell, James R Kozloski, Baljit S Khakh

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.

0numbers the graph read from it
0cells of the map it votes in
62citing papers in PubMed
5.7field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

62 citing papers in PubMed, 99 citations in OpenAlex.

  1. Dilating the aging clock with light.Innovation (Cambridge (Mass.)) · 2026
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  15. Astrocytes in Rodent Anxiety-Related Behavior: Role of Calcium and Beyond.International journal of molecular sciences · 2025
    Review
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  19. Article
  20. Astrocyte Calcium Signaling.Cold Spring Harbor perspectives in biology · 2024
    Review

2 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

J Christopher OcteauDepartment of Physiology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095-1751, USA.
Mohitkumar R GangwaniDepartment of Physiology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095-1751, USA.
Sushmita L AllamIBM T.J. Watson Research Center, P.O. Box 218, 1101 Kitchawan Road, Yorktown Heights, NY 10598, USA.
Duy TranDepartment of Neurology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095-1751, USA.
Shuhan HuangDepartment of Physiology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095-1751, USA.
Tuan M Hoang-TrongIBM T.J. Watson Research Center, P.O. Box 218, 1101 Kitchawan Road, Yorktown Heights, NY 10598, USA.
Peyman GolshaniDepartment of Neurology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095-1751, USA; Department of Psychiatry and Biobehavioral Sciences, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095-1751, USA; Intellectual and Developmental Disabilities Research Center, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095-1751, USA; Center for Neurobehavioral Genetics, Semel Institute for Neuroscience and Human Behavior, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095-1751, USA.
Timothy H RumbellIBM T.J. Watson Research Center, P.O. Box 218, 1101 Kitchawan Road, Yorktown Heights, NY 10598, USA.
James R KozloskiIBM T.J. Watson Research Center, P.O. Box 218, 1101 Kitchawan Road, Yorktown Heights, NY 10598, USA.
Baljit S KhakhDepartment of Physiology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095-1751, USA; Department of Neurobiology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095-1751, USA. Electronic address: bkhakh@mednet.ucla.edu.
University of California, Los Angeles · USIBM (United States) · US

Funding

UCLA MSD ProgramR25GM055052 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI BARBER, PAUL HENRY, HASSON, TAMA W. · 1996 to 2021
$9.2M
Fundamental astrocyte biology in intact neural circuitsR35NS111583 · NINDS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Baljit Khakh · 2019 to 2026
$7.9M
Functions of astrocyte calcium signaling in the striatumR01NS060677 · NINDS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI KHAKH, BALJIT · 2008 to 2018
$4.3M
Astrocyte branchlet dysfunction as an early step in brain disordersDP1MH104069 · NIMH · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI KHAKH, BALJIT · 2013 to 2017
$3.9M
New Optical and Genetic Tools to Study Diverse Calcium Signals in AstrocytesR01MH099559 · NIMH · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI KHAKH, BALJIT · 2013 to 2017
$1.9M
NIGMS NIH HHS R25 GM055052NIMH NIH HHS DP1 MH104069NIMH NIH HHS R01 MH099559NINDS NIH HHS R01 NS060677NINDS NIH HHS R35 NS111583
6 · The paper itself

Abstract

Channelrhodopsin2 (ChR2) optogenetic excitation is widely used to study neurons, astrocytes, and circuits. Using complementary approaches in situ and in vivo, we found that ChR2 stimulation leads to significant transient elevation of extracellular potassium ions by ∼5 mM. Such elevations were detected in ChR2-expressing mice, following local in vivo expression of ChR2(H134R) with adeno-associated viruses (AAVs), in different brain areas and when ChR2 was expressed in neurons or astrocytes. In particular, ChR2-mediated excitation of striatal astrocytes was sufficient to increase medium spiny neuron (MSN) excitability and immediate early gene expression. The effects on MSN excitability were recapitulated in silico with a computational MSN model and detected in vivo as increased action potential firing in awake, behaving mice. We show that transient, physiologically consequential increases in extracellular potassium ions accompany ChR2 optogenetic excitation. This coincidental effect may be important to consider during astrocyte studies employing ChR2 to interrogate neural circuits and animal behavior.

Indexed as

AnimalsChannelrhodopsinsMiceOptogeneticsPotassiumChannelrhodopsinsPotassiumastrocytechannelrhodopsincircuitneuronoptogeneticspotassiumstriatum

Identifiers

PMID31116972
PMCPMC6582980
OpenAlexW2945799935

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.