ArticlePloS one2019
Comparison of TaqMan, KASP and rhAmp SNP genotyping platforms in hexaploid wheat.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- Development and validation of KASP markers for powdery mildew resistance in Korean naked barley using QTL-seq.Molecular breeding : new strategies in plant improvement · 2026Article
- Immune gene diversity and STING1 variants in shaping cancer immunity across different genetic ancestry populations.Cell reports · 2026Article
- Development and application of duplex and triplex assays for simultaneous detection of resistance genes to leaf rust, Fusarium head blight, powdery mildew, Septoria tritici blotch, eyspot, stem rust and yellow rust in wheat.Journal of applied genetics · 2026Article
- Development and application of SNP markers to discriminate Korean Perilla (Perilla frutescens) varieties using genomic sequence variations.Scientific reports · 2025Article
- Suitability of Real-Time PCR Methods for New Genomic Technique Detection in the Context of the European Regulations: A Case Study in Arabidopsis.International journal of molecular sciences · 2025Article
- Article
- Development and validation of a minimal SNP genotyping panel for the differentiation of Cannabis sativa cultivars.BMC genomics · 2025Article
- Article
- SNP loci identification and KASP marker development system for genetic diversity, population structure, and fingerprinting in sweetpotato (Ipomoea batatas L.).BMC genomics · 2024Article
- Accurate and cost-effective generation of a genetic risk score direct from blood lysates.Journal of translational medicine · 2024Article
- Allele-tagged TaqManMolecular biology reports · 2024Article
- Genotyping single point mutations in rd1 and rd8 mice using melting curve analysis of qPCR fragments.Scientific reports · 2024Article
- KASP: a high-throughput genotyping system and its applications in major crop plants for biotic and abiotic stress tolerance.Molecular biology reports · 2024Review
- History and prospects of flax genetic markers.Frontiers in plant science · 2024Review
- Development of KASP markers, SNP fingerprinting and population genetic analysis ofFrontiers in plant science · 2024Article
- The Past, Present, and Future of Wheat Dwarf Virus Management-A Review.Plants (Basel, Switzerland) · 2023Review
- Automating high-throughput screening for anthracnose resistance in common bean using allele specific PCR.Plant methods · 2023Article
- Detection and distribution of two dominant alleles associated with the sweet kernel phenotype in almond cultivated germplasm.Frontiers in plant science · 2023Article
- KASP-IEva: an intelligent typing evaluation model for KASP primers.Frontiers in plant science · 2023Article
- Genetic approaches to exploit landraces for improvement ofFrontiers in plant science · 2023Review
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Advances in high-throughput genotyping enable the generation of genome-scale data much more easily and at lower cost than ever before. However, small-scale and cost-effective high-throughput single-nucleotide polymorphism (SNP) genotyping technologies are still under development. In this study, we compared the performances of TaqMan, KASP and rhAmp SNP genotyping platforms in terms of their assay design flexibility, assay design success rate, allele call rate and quality, ease of experiment run and cost per sample. Fifty SNP markers linked to genes governing various agronomic traits of wheat were chosen to design SNP assays. Design success rates were 39/50, 49/50, and 49/50 for TaqMan, KASP, and rhAmp, respectively, and 30 SNP assays were manufactured for genotyping comparisons across the three platforms. rhAmp showed 97% of samples amplified while TaqMan and KASP showed 93% and 93.5% of amplifications, respectively. Allele call quality of rhAmp was 97%, while it was 98% for both TaqMan and KASP. rhAmp and KASP showed significantly better (p < 0.001) allele discrimination than TaqMan; however, TaqMan showed the most compact cluster. Based on the current market, rhAmp was the least expensive technology followed by KASP. In conclusion, rhAmp provides a reliable and cost-effective option for targeted genotyping and marker-assisted selection in crop genetic improvement.
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